Transcriptomics,Genomics

Dataset Information

26

Expression data from graft infiltrating macrophages treated with mTORi-HDL nanobiologics


ABSTRACT: Inducing graft acceptance without chronic immunosuppression remains an elusive goal in organ transplantation. Recent data demonstrate that non-self recognition by graft infiltrating macrophages initiates transplant rejection. Using an experimental transplantation mouse model, we isolated graft-infiltrating macrophages from two transplantation settings: untreated rejecting mice and treated with mTORi-HDL nanobiologics. We used microarrays to detail the global programme of gene expression underlying macrophage dependent organ transplant rejection and identified distinct classes of up-regulated genes during this process, which are down-regulated following tolerogenic treatment with mTORi-HDL nanobiologics. Overall design: BALB/c donor hearts were transplanted into fully allogeneic C57BL/6 mice. Transplant recipients were either left untreated (Control) and rejected their allografts or were treated with mTORi-HDL nanobiologics (Sample) on days 0, 2, and 5 post-transplantation. Donor heart allografts were isolated on day 6 post-transplantation and graft-infiltrating macrophages were isolated by fluorescence-activated cell sorting (FACS) for microarray analysis.

INSTRUMENT(S): [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]

SUBMITTER: Jordi Ochando  

PROVIDER: GSE119370 | GEO | 2018-10-31

REPOSITORIES: GEO

Similar Datasets

2013-10-04 | E-GEOD-50058 | ArrayExpress
2010-07-06 | GSE16695 | GEO
2010-07-29 | E-GEOD-16695 | ArrayExpress
2010-06-10 | E-GEOD-2445 | ArrayExpress
2005-09-01 | GSE2445 | GEO
2014-10-21 | E-GEOD-52164 | ArrayExpress
| GSE88845 | GEO
2012-12-30 | E-GEOD-32545 | ArrayExpress
2014-06-01 | E-GEOD-53605 | ArrayExpress
| GSE88844 | GEO