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Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses (Experiment 1: RNA-seq)


ABSTRACT: By taking advantage of the highly immunogenic live-attenuated yellow fever virus vaccine YFV-17D, we performed an in-depth comparison of transcriptomic responses in human vaccinees, conventional humanized mice, and second generation humanized mice. We demonstrate that selective expansion of human myeloid and natural killer cells in humanized mice promotes transcriptomic responses akin to those of human vaccinees Overall design: The transcriptome of human PBMCs from different humanized mouse models (conventional model: 3 cohorts/samples of 5 mice each; NFA2-HIS/Fluc model: 2 cohorts/samples of 4 mice each; NFA2-HIS/Flt3LG model: 3 cohorts/samples of 4 mouse each) was determined by RNA-Seq prior (day 0 post infection; control) and after YFV-17D infection (day 11 post infection; post infection). Resulting data sets of differentially expressed genes were compared to data sets from human vaccinees (GSE13485 and GSE13699). Total number of samples processed for RNA-seq: 16

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

ORGANISM(S): Homo Sapiens

SUBMITTER: Alexander Ploss  

PROVIDER: GSE119749 | GEO | 2018-11-28

REPOSITORIES: GEO

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Selective expansion of myeloid and NK cells in humanized mice yields human-like vaccine responses.

Douam Florian F   Ziegler Carly G K CGK   Hrebikova Gabriela G   Fant Bruno B   Leach Robert R   Parsons Lance L   Wang Wei W   Gaska Jenna M JM   Winer Benjamin Y BY   Heller Brigitte B   Shalek Alex K AK   Ploss Alexander A  

Nature communications 20181128 1


Mice engrafted with components of a human immune system have become widely-used models for studying aspects of human immunity and disease. However, a defined methodology to objectively measure and compare the quality of the human immune response in different models is lacking. Here, by taking advantage of the highly immunogenic live-attenuated yellow fever virus vaccine YFV-17D, we provide an in-depth comparison of immune responses in human vaccinees, conventional humanized mice, and second gene  ...[more]

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