Genomics

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Nut/Hos1 and Sas2/Rpd3 control H3 acetylation of two different sets of osmotic stress-induced genes


ABSTRACT: Epigenetic information is able to interact with the cellular environment and therefore it could be especially useful for reprogramming gene expression in response to a physiological perturbation. In fact, genes induced or repressed by osmotic stress undergo significant changes in the levels of various histone modifications, especially the acetylation levels of histone H3. Exposure of yeast to high osmolarity results in the activation of p38-related SAPK, Hog1, which plays a central role in the control of gene expression. Therefore, two types of mechanisms converge in gene regulation by stress: that of the epigenetic signals and that of the trans-acting protein factor. We have evaluated the connection between the presence of Hog1 and the changes on acetylation of histone H3 in stress-regulated genes. Furthermore, we asked which enzymatic activities are involved in the changes produced in the acetylation of H3 linked to gene activation by osmotic stress. We have demonstrated that increase of acetylation of lysines 9 and 14 of H3 produced on induced genes during stress positively correlates between them and are largely dependent on Hog1 at genome-wide level. Conversely, decrease in acetylation on repressed genes was not dependent on Hog1. However, the absence of Hog1 produces different and even opposite effects on the induction and acetylation of H3 of each gene, which suggests that Hog1 influences both processes through different mechanisms. Additionally, we found that in genes up-regulated during osmotic stress in a Msn2/Msn4-independent manner, the state of acetylation of lysine 9 of H3 was altered in strains deficient in Nut1 HAT and Hos1 HDAC; meanwhile for Msn2/Msn4-dependent induced genes H3k9 acetylation varies in strains deficient in Sas2 HAT and Rpd3 HDAC. During induction by osmotic stress at least two different mechanism act on H3k9 acetylation of two differentiated set of induced genes, one of them would act through Nut1/Hos1, while the other through Sas2/Rpd3. The results presented here show new and unexpected participants in the process of the regulation of gene in response to environmental perturbations. Overall design: Histone H3 acetylation at lysines K9 and K14 respect signal for total H3 protein, both measured by ChIP, in wt (BY4742) and hog1delta yeast cells under non-stress and 10 min of 0.4 M NaCl treatment. One replica experiment. The hog1Δ data obtained in this study was compared to the wild type data obtained by Magraner-Pardo et al., 2014 (GSE41587).

INSTRUMENT(S): Agilent-014741 Yeast Whole Genome ChIP-on-Chip Microarray 244K (G4491A)

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Paula Alepuz  

PROVIDER: GSE127742 | GEO | 2019-07-01

REPOSITORIES: GEO

Dataset's files

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Action DRS
GSE127742_Processed_data_H3K14.xlsx Xlsx
GSE127742_Processed_data_H3K9.xlsx Xlsx
GSE127742_RAW.tar Raw
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