Dataset Information


Global gene expression changes including drug metabolism and disposition induced by three-dimensional culture of HepG2

ABSTRACT: We found constitutive upregulation and higher degree induction of drug metabolism and disposition-related genes in a three-dimensional HepG2 culture. The upregulated genes are those believed to be regulated by different regulatory factors. The global gene expression analysis by Affymetrix GeneChip indicated that altered expressions of microtubule-related genes may change expressed levels of drug metabolism and disposition genes. Stabilization of the microtubule molecules with docetaxel, a tubulin stabilizing agent, in the two-dimensional culture showed gene expression patterns similar to those in the three-dimensional culture, indicating that culture environment affects drug metabolism functions in HepG2 cells. Keywords: radial flow bioreactor cell culture system, three dimensional culture, HepG2, GeneChip U133A, Overall design: We compared the gene expression data from HepG2 cells cultured in the radial flow bioreactor (RFB) cell culture system for six days to those from HepG2 cells cultured in tissue culture plates for five days. Both cell cultures (RFB culture and plate culture) were performed twice independently. In each culture, three samples were collected from three different portions of the bioreactor or from three different tissue culture plates. Microarray analyses were performed in duplicate for each sample using the Affymetrix human genome U133A GeneChip.

INSTRUMENT(S): [HG-U133A] Affymetrix Human Genome U133A Array

ORGANISM(S): Homo sapiens  

SUBMITTER: Seiichi Ishida  

PROVIDER: GSE12939 | GEO | 2009-02-24



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