Project description:Transcription profiling of Arabidopsis plants grown in high versus low Cu2+ concentration. Keywords: Stress response Three replicates for each sample category (Arabidopsis Col0 grown either in high or low Cu2+) were generated, and compared directly to each other, in a non-paired direct comparisson. Two technical replicas from each biological replica were carried out.
Project description:Arabidopsis thaliana is a glycophyte with a low salt tolerance, while Eutrema is a halophyte with a very high salt tolerance. To elucidate the transcriptional basis of this difference, we performed hydroponis culture experiments where we grew plants under control conditions (25 mM NaCl) or under salt stress (200 mM NaCl for both species, 500 mM for Eutrema). Salt concentration was increased for the stress treatments by increments of 50 mM per day (25 mM on the first day). Plants were grown at the final NaCl concentration for an additional week, when rosettes were harvested for RNA isolation.Expression patterns were compared between treatments and between species.
Project description:ra05-12_nar2 - atnar2.1-1 - Does the ko of AtNAR2.1 leads to a differential expression in comparison to the WT when grown under high nitrate condition and transfered onto low nitrate concentration? - Plants were grown for 41 days in hydroponics in media containing 6 mM nitrate and then transferred to 6 or 0.2 mM nitrate media for 24h ( short days, irradiation 150 uE). Plants were harvested on day 42 during the first 2 hours of light. Keywords: dose response,gene knock out
Project description:Transcriptional profiling of 60h-old Arabidopsis whole seedlings comparing control Col-0 wild-type plants with pifQ mutant plants The expression profile of dark-grown pifQ mutant shows similar pattern of Rc-grown Col-0 wild-type Keywords: Genetic modification
Project description:We used N-(1-naphthyl) phthalamic acid (NPA)-induced vascular overgrowth in Arabidopsis leaves to look for differential up-regulation of genes in NPA-treated tissues that may be involved in vascular differentiation. Arabidopsis thaliana Col-0 plants were grown for approximately 2 weeks on solid ATS medium (1) containing a final concentration of 10 um NPA (dissolved in DMSO) or an equivalent volume of DMSO (control). At this stage plants had approximately 6 rosette leaves. RNA was prepared from entire shoot tissues of control (DMSO) or NPA-treated plants.(1) Lincoln et al., 1990. Plant Cell 2: 1071-1080.
Project description:To develop a screening system for plant activators, which are novel substances that protect plants by enhancing their inherent disease-resistance mechanisms, we performed analysis using an Arabidopsis microarray consisting of 1200 full-length cDNA clones representing putative defense-related and regulatory genes. A total of 1.2K potential biotic and abiotic stress-related genes were selected from the genes covered by the Arabidopsis 7K array (RIKEN, Japan) and Arabidopsis oligo microarray (Agilent Technologies, USA) for this study. Arabidopsis wild-type plants (ecotype Columbia; Col-0) were grown in soil for 28 days in a growth chamber at 22。C under a 12-h light/ 12-h dark cycle. Arabidopsis plants were applied a foliar spray with 5 mM SA, 0.1 mM MeJA, 1 mM ethephon, 0.5 mM BTH, 10 mM BABA and 1 mM INA. Benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester and 2,6-dichloroisonicotinic acid activated plant defense responses via the salicylic acid (SA)-dependent signaling pathway, and _-aminobutyric acid triggered a primed state in the plant that enables more efficient activation of the SA-, jasmonic acid- and ethylene-signaling pathway. These results suggest that this novel system can be used to screen for candidate plant activators. Keywords: time course, dose response
Project description:The goal of the experiments is to identify differential genes between the roots of Arabidopsis thaliana and roots treated for 3 h with 1-aminocyclopropane-1-carboxylic acid (ACC). ACC blocks the maximal cell elongation of the root cells. - Arabidopsis plants are grown for 5 days on MS medium and then we transfer them to a medium supplemented with ACC (5uM) or without ACC. After 3 h of growth, we isolate the RNA of the roots. Keywords: treated vs untreated comparison
Project description:Series of 10 repetitions of hybridization of treatment (mCO2_4d) and control (0d) each. Comparison of plants grown for 96 h at low-CO2 (0.003 % (v/v) CO2) versus plants grown at normal CO2 level. E. Richly et al., EMBO Rep. 4 (2003), pp. 491–498 Keywords: repeat sample
Project description:Series of 6 repetitions of hybridization of treatment (mCO2_2d) and control (0d) each. Comparison of plants grown for 48 h at low-CO2 (0.003 % (v/v) CO2) versus plants grown at normal CO2 level. E. Richly et al., EMBO Rep. 4 (2003), pp. 491–498 Keywords: repeat sample