Genomics

Dataset Information

22

A role for mammalian Sin3 in permanent gene silencing


ABSTRACT: The multi-subunit Sin3 co-repressor complex regulates gene transcription through deacetylation of nucleosomes. However, the full range of Sin3 activities and targets is not well understood. Here, we have investigated genome-wide binding of mouse Sin3 and RBP2 as well as histone modifications and nucleosome positioning as a function of myogenic differentiation. Remarkably, we find that Sin3 complexes spread immediately downstream of the transcription start site on repressed and transcribed genes during differentiation. We show that RBP2 is part of a Sin3 complex, and on a subset of E2F4 target genes, the coordinated activity of Sin3 and RBP2 leads to deacetylation, demethylation, and repositioning of nucleosomes. Our work provides evidence for coordinated binding of Sin3, chromatin modifications, and chromatin remodeling within discrete regulatory regions, suggesting a model in which spreading of Sin3 binding is ultimately linked to permanent gene silencing on a subset of E2F4 target genes. Overall design: Factor binding on genome-wide promoter arrays were performed at least in duplicate. Factor binding on high density tiling arrays were performed at least in triplicate. Histone modifications and nucleosome density profiles were performed at least in duplicate

INSTRUMENT(S): Agilent-014716 Mouse Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 (G4490A) (Feature Number version)

SUBMITTER: Brian Dynlacht  

PROVIDER: GSE13247 | GEO | 2008-11-06

SECONDARY ACCESSION(S): PRJNA109527

REPOSITORIES: GEO

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Publications

A role for mammalian Sin3 in permanent gene silencing.

van Oevelen Chris C   Wang Jinhua J   Asp Patrik P   Yan Qin Q   Kaelin William G WG   Kluger Yuval Y   Dynlacht Brian David BD  

Molecular cell 20081101 3


The multisubunit Sin3 corepressor complex regulates gene transcription through deacetylation of nucleosomes. However, the full range of Sin3 activities and targets is not well understood. Here, we have investigated genome-wide binding of mouse Sin3 and RBP2 as well as histone modifications and nucleosome positioning as a function of myogenic differentiation. Remarkably, we find that Sin3 complexes spread immediately downstream of the transcription start site on repressed and transcribed genes du  ...[more]

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