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Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus Rattus norvegicus Homo sapiens

PROVIDER: GSE14031 | GEO | 2009/07/22

SECONDARY ACCESSION(S): PRJNA112457

REPOSITORIES: GEO

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Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo

Project description:This SuperSeries is composed of the following subset Series: GSE13858: Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment GSE13859: Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice Refer to individual Series

2010-06-24 | E-GEOD-14031 | biostudies-arrayexpress

Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice

Project description:To examine the effect of estrogen receptor alpha for the miRNA expression, total RNA extracted from whole embryos at E18.5 of wild type and Estrogen Receptor alpha knock-out mice

2009-07-22 | GSE13859 | GEO

Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice

2010-06-24 | E-GEOD-13859 | biostudies-arrayexpress

Global survey of miRNA Taqman RT-PCR of whole embryo: wild-type vs. estrogen receptor alpha knockout mice

2009-07-22 | GSE16513 | GEO

Global survey of miRNA Taqman RT-PCR of whole embryo: wild-type vs. estrogen receptor alpha knockout mice

2009-07-22 | E-GEOD-16513 | biostudies-arrayexpress

Disruption of estrogen signaling does not prevent progesterone action in the estrogen receptor alpha knockout mouse uterus.

Project description:Estrogen is known to increase progesterone receptor (PR) levels in the wild-type mouse uterus, and this estrogen induction was thought to be important for progesterone action through the PR. The estrogen receptor alpha knockout (ERKO) mouse uterus was observed to express PR mRNA that cannot be induced by estrogen. Progesterone action was characterized to determine whether it was diminished in ERKO mice. The PR protein is present in the ERKO uterus at 60% of the level measured in a wild-type uterus. The PR-A and PR-B isoforms are both detected on Western blot, and the ratio of isoforms is the same in both genotypes. Although the level of PR is reduced in the ERKO uterus, the receptor level is sufficient to induce genomic responses, since both calcitonin and amphiregulin mRNAs were increased after progesterone treatment. Finally, the ERKO uterus can be induced to undergo a progesterone-dependent decidual response. Surprisingly, the decidual response is estrogen independent in the ERKO, although it remains estrogen dependent in a wild type. These results indicate that estrogen receptor alpha modulation of PR levels is not necessary for expression of the PR or genomic and physiologic responses to progesterone in the ERKO uterus.

| S-EPMC22348 | biostudies-literature

Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment

Project description:To examine the effect of E2 treatment for the miRNA expression, at 15 week old, female wiled type mice were ovariectomized, and after one week, estradiol (E2) was delivered at a concentration of 0.050 mg/kg body weight/day. 24 hours after chemical treatment, uteruses from mice treated with or without E2 were dissected.

2009-07-22 | GSE13858 | GEO

Oviductal estrogen receptor α signaling prevents protease-mediated embryo death.

Project description:Development of uterine endometrial receptivity for implantation is orchestrated by cyclic steroid hormone-mediated signals. It is unknown if these signals are necessary for oviduct function in supporting fertilization and preimplantation development. Here we show that conditional knockout (cKO) mice lacking estrogen receptor α (ERα) in oviduct and uterine epithelial cells have impaired fertilization due to a dramatic reduction in sperm migration. In addition, all successfully fertilized eggs die before the 2-cell stage due to persistence of secreted innate immune mediators including proteases. Elevated protease activity in cKO oviducts causes premature degradation of the zona pellucida and embryo lysis, and wild-type embryos transferred into cKO oviducts fail to develop normally unless rescued by concomitant transfer of protease inhibitors. Thus, suppression of oviductal protease activity mediated by estrogen-epithelial ERα signaling is required for fertilization and preimplantation embryo development. These findings have implications for human infertility and post-coital contraception.

| S-EPMC4718728 | biostudies-literature

Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment

2010-06-24 | E-GEOD-13858 | biostudies-arrayexpress

Serine 216 phosphorylation of estrogen receptor α in neutrophils: migration and infiltration into the mouse uterus.

Project description:BackgroundWhereas estrogen receptors are present in immune cells, it is not known if they are phosphorylated to regulate immune cell functions. Here we determined the phosphorylation status of estrogen receptor α (ERα) at residue serine 216 in mouse neutrophils and examined its role in migration and infiltration. Serine 216 is the conserved phosphorylation site within the DNA binding domains found in the majority of nuclear receptors.Methodology/principal findingsA phospho-peptide antibody specific to phosphorylated serine 216 and ERα KO mice were utilized in immunohistochemistry, double immuno-staining or Western blot to detect phosphorylation of ERα in peripheral blood as well as infiltrating neutrophils in the mouse uterus. Transwell assays were performed to examine migration of neutrophils. An anti-Ly6G antibody identified neutrophils. About 20% of neutrophils expressed phosphorylated ERα at serine 216 in peripheral white blood cells (WBC) from C3H/HeNCrIBR females. Phosphorylation was additively segregated between C3H/HeNCrIBR and C57BL/6 females. Only neutrophils that expressed phosphorylated ERα migrated in Transwell assays as well as infiltrated the mouse uterus during normal estrous cycles.Conclusions/significanceERα was phosphorylated at serine 216 in about 20% of mouse peripheral blood neutrophils. Only those that express phosphorylated ERα migrate and infiltrate the mouse uterus. This phosphorylation was the first to be characterized in endogenous ERα found in normal tissues and cells. Phosphorylated ERα may have opened a novel research direction for biological roles of phosphorylation in ERα actions and can be developed as a drug target for treatment of immune-related diseases.

| S-EPMC3873424 | biostudies-literature

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