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Omics score: 20

Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Overall design: Refer to individual Series

INSTRUMENT(S): FilgenArray miRNA384

SUBMITTER: kaoru yamagata

PROVIDER: GSE14031 | GEO | 2009-07-22

SECONDARY ACCESSION(S): PRJNA112457 GSE13858 GSE13859

REPOSITORIES: GEO

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	GSE14031_RAW.tar	Raw
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Global survey of miRNA profiles in the uterus and an estrogen receptor alpha knockout embryo

Project description:This SuperSeries is composed of the following subset Series: GSE13858: Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment GSE13859: Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice Refer to individual Series

2010-06-24 | E-GEOD-14031 | ArrayExpress

Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice

Project description:To examine the effect of estrogen receptor alpha for the miRNA expression, total RNA extracted from whole embryos at E18.5 of wild type and Estrogen Receptor alpha knock-out mice Overall design: Two group experiment (wild type and ER alpha KO)

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Global survey of miRNA microarray of whole embryo, wild type vs estrogen receptor alpha knockout mice

2010-06-24 | E-GEOD-13859 | ArrayExpress

Global survey of miRNA Taqman RT-PCR of whole embryo: wild-type vs. estrogen receptor alpha knockout mice

2009-07-22 | GSE16513 | GEO

Global survey of miRNA Taqman RT-PCR of whole embryo: wild-type vs. estrogen receptor alpha knockout mice

2009-07-22 | E-GEOD-16513 | ArrayExpress

Disruption of estrogen signaling does not prevent progesterone action in the estrogen receptor alpha knockout mouse uterus.

Project description:Estrogen is known to increase progesterone receptor (PR) levels in the wild-type mouse uterus, and this estrogen induction was thought to be important for progesterone action through the PR. The estrogen receptor alpha knockout (ERKO) mouse uterus was observed to express PR mRNA that cannot be induced by estrogen. Progesterone action was characterized to determine whether it was diminished in ERKO mice. The PR protein is present in the ERKO uterus at 60% of the level measured in a wild-type uterus. The PR-A and PR-B isoforms are both detected on Western blot, and the ratio of isoforms is the same in both genotypes. Although the level of PR is reduced in the ERKO uterus, the receptor level is sufficient to induce genomic responses, since both calcitonin and amphiregulin mRNAs were increased after progesterone treatment. Finally, the ERKO uterus can be induced to undergo a progesterone-dependent decidual response. Surprisingly, the decidual response is estrogen independent in the ERKO, although it remains estrogen dependent in a wild type. These results indicate that estrogen receptor alpha modulation of PR levels is not necessary for expression of the PR or genomic and physiologic responses to progesterone in the ERKO uterus.

1999-01-01 | S-EPMC22348 | BioStudies

Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment

Project description:To examine the effect of E2 treatment for the miRNA expression, at 15 week old, female wiled type mice were ovariectomized, and after one week, estradiol (E2) was delivered at a concentration of 0.050 mg/kg body weight/day. 24 hours after chemical treatment, uteruses from mice treated with or without E2 were dissected. Overall design: Two group experiment (WT-OVX and WT-OVX-E2) three replicates per condition

2009-07-22 | GSE13858 | GEO

Global survey of miRNA microarray of uterus, ovariectomized female mice with or without estrogen (E2) treatment

Project description:To examine the effect of E2 treatment for the miRNA expression, at 15 week old, female wiled type mice were ovariectomized, and after one week, estradiol (E2) was delivered at a concentration of 0.050 mg/kg body weight/day. 24 hours after chemical treatment, uteruses from mice treated with or without E2 were dissected. Two group experiment (WT-OVX and WT-OVX-E2) three replicates per condition

2010-06-24 | E-GEOD-13858 | ArrayExpress

Global transcriptome analysis of mouse uterus during embryo implantation by SAGE

Project description:The aim of this study was to examine differential gene expression profile in mouse uterus on day 5 of pregnancy between implantation sites and inter-implantation sites. There are 104,520 tags sequenced and acquired, 51,306 for non-implantation site, and 53,214 for implantation site. Keywords: Implantation, Uterus, Differential gene expression, Implantation sites and inter-implantation sites were isolated from the mouse uteri on day 5 of pregnancy from at least 20 mice. Implantation sites were identified by tail-vein injection of 0.1 ml of 1% Chicago blue 5 min before sample collection. SAGE analysis was used to examine differential gene expression in mouse uterus during embryo implantation.

2005-07-13 | E-GEOD-2892 | BioStudies

Complete knockout of estrogen receptor alpha is not directly protective in murine lupus.

Project description:Systemic lupus erythematosus (SLE) is a chronic and potentially severe autoimmune disease that disproportionately affects women. Despite a known role for hormonal factors impacting autoimmunity and disease pathogenesis, the specific mechanisms of action remain poorly understood. Our laboratory previously backcrossed "estrogen receptor alpha knockout (ER?KO)" mice onto the NZM2410 lupus prone background to generate NZM/ER?KO mice. This original ER?KO mouse, developed in the mid-1990s and utilized in hundreds of published studies, is not in fact ER? null. They express an N-terminally truncated ER?, and are considered a functional KO. They have physiologic deficiencies including infertility due to disruption of a critical activation domain (AF-1) at the N terminus of ER?, required for most classic estrogen (E2) actions. We demonstrated that female NZM/ER?KO mice had significantly less renal disease and significantly prolonged survival compared to WT littermates despite similar serum levels of autoantibodies and glomerular immune complex deposition. Herein, we present results of experiments using a lupus prone true ER?-/- mice (deletional KO mice on the NZM2410 background), surprisingly finding that these animals were not protected if they were ovariectomized, suggesting that another hormonal component confers protection, possibly testosterone, rather than the absence of the full-length ER?.

2017-01-01 | S-EPMC6261466 | BioStudies

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