Transcriptomics

Dataset Information

0

MiR-378a-3p is critical for Burkitt lymphoma cell growth


ABSTRACT: To gain further knowledge on the role of microRNAs (miRNAs) in the pathogenesis of Burkitt lymphoma (BL), we performed small RNA sequencing in BL cell lines and normal germinal center B (GC-B) cells. This revealed 26 miRNAs with significantly different expression levels. Altered levels were validated by qRT-PCR for six of eight selected miRNAs. For five miRNAs, the differential expression pattern was confirmed in BL primary tissues compared to GC-B cells. Inhibition of miR-378a-3p reduced the growth of multiple BL cell lines. RNA immunoprecipitation of Argonaute 2 followed by microarray analysis (Ago2-RIP-Chip) upon inhibition and ectopic overexpression of miR-378a-3p revealed 63 and 20 putative miR-378a-3p targets, respectively. These included 28 genes with a putative miR-378a-3p binding site. Effective targeting by miR-378a-3p was confirmed by luciferase reporter assays for four out of eight selected genes: MNT, FOXP1, IRAK4, and lncRNA JPX, all of which have been implicated in proliferation and cancer. In summary, our study identified several differentially expressed miRNAs in BL. We identified miR-378a-3p as a miRNA with an oncogenic role in BL and revealed 4 novel miR-378a-3p target genes, i.e. MNT, FOXP1, IRAK4, and JPX.

ORGANISM(S): Homo sapiens

PROVIDER: GSE141691 | GEO | 2020/06/30

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-02-22 | GSE107088 | GEO
2021-10-06 | GSE143740 | GEO
2021-03-08 | GSE164400 | GEO
2023-01-26 | GSE223538 | GEO
| EGAD00001006842 | EGA
| EGAS00001005296 | EGA
2014-06-01 | GSE56268 | GEO
2022-07-25 | GSE209568 | GEO
| PRJNA418978 | ENA
| EGAD00001006844 | EGA