Dataset Information


Genome-wide analysis of expression and DNA methylation at CpG-island-associated sense-antisense transcript loci in mouse

ABSTRACT: Sense-antisense transcript (SAT) pairs are pairs of transcripts that fully or partially overlap but are transcribed in opposite directions. Although SAT expression occurs in various species, most SAT pairs have not been examined in detail. Because our previous studies revealed some tissue specificity in SAT expression, SAT pairs might be involved in cell differentiation and the maintenance of tissue-specific gene expression programs. To analyze such tissue-specific SAT pairs, we assessed the expression profiles of SATs from 12 tissues of normal mice at a genome-wide scale and found that a considerable number of SAT pairs showed expression patterns unique to testis. This finding prompted us to study the relationship between SAT expression pattern and another epigenetic gene regulatory mechanism, DNA methylation. We conducted a comparative global analysis of the DNA methylation status of CpG island (CGI)-associated SAT loci from various tissues and found that in 99 of the 4911 SAT pairs studied, DNA methylation could cooperatively suppress its downstream “sense” transcription together with the “antisense” transcriptional activity in a tissue-specific manner. The tissue-specific differentially methylated regions (T-DMRs) of these SAT pairs mainly occurred outside of the defined CGIs. In addition, some of these T-DMRs are situated at the 5′ region of the antisense transcripts. This positioning implies a novel mechanism for DNA methylation to regulate gene transcription, in which DNA methylation inhibits an opposing transcriptional activity and therefore maintains stable expression of a tissue-specific gene. Overall design: Twelve tissues (brain, thymus, heart, lung, liver, spleen, stomach, kidney, small intestine, testis, and placentae [10.5 and 13.5 days postcoitum (dpc)]) of C57BL/6J Jc1 and a mouse fibroblast cell line (SL10) were used for the mouse expression oligo-microarray. The expression array data were obtained by both oligo-dT and random priming methods, except for SL10 (olio-dT only). Twelve tissues (brain, thymus, heart, lung, liver, spleen, stomach, kidney, small intestine, testis, and placentae [10.5 and 13.5 days postcoitum (dpc)]) were used for DIP chip experiments. Nine of twelve tissues were separately analyzed classified on the basis of sex. A technical duplication of DIP chip analysis was performed by using the same genomic DNA of male kidney.

INSTRUMENT(S): Agilent Mouse 44k Sense-Antisense Transcript Microarray

ORGANISM(S): Mus musculus  

SUBMITTER: Yutaka Watanabe  

PROVIDER: GSE14392 | GEO | 2010-08-26



Similar Datasets

2010-08-26 | E-GEOD-14392 | ArrayExpress
2008-02-20 | GSE9581 | GEO
2010-06-25 | E-GEOD-9581 | ArrayExpress
2012-12-10 | E-GEOD-38291 | ArrayExpress
| GSE38291 | GEO
| phs000937 | dbGaP
2011-03-01 | GSE14397 | GEO
2011-03-01 | E-GEOD-14397 | ArrayExpress
2011-03-01 | E-GEOD-14398 | ArrayExpress
2011-03-01 | GSE14398 | GEO