Genomics

Dataset Information

0

Gene expression profiling of lymphoblastoid cell lines from autistic and nonaffected sib pairs


ABSTRACT: Despite the identification of numerous autism susceptibility genes, the pathobiology of autism remains unknown. The present “case-control” study takes a global approach to understanding the molecular basis of autism spectrum disorders based upon large-scale gene expression profiling. DNA microarray analyses were conducted on lymphoblastoid cell lines from over 20 sib pairs in which one sibling had a diagnosis of autism and the other was not affected in order to identify biochemical and signaling pathways which are differentially regulated in cells from autistic and nonautistic siblings. Bioinformatics and gene ontological analyses of the data implicate genes which are involved in nervous system development, inflammation, and cytoskeletal organization, in addition to genes which may be relevant to gastrointestinal or other physiological symptoms often associated with autism. Moreover, the data further suggests that these processes may be modulated by cholesterol/steroid metabolism, especially at the level of androgenic hormones. Elevation of male hormones, in turn, has been suggested as a possible factor influencing susceptibility to autism, which affects ~4 times as many males as females. Metabolic profiling of steroid hormones in lymphoblastoid cell lines from several pairs of siblings reveals higher levels of testosterone in the autistic sibling, which is consistent with the increased expression of two genes involved in the steroidogenesis pathway. Global gene expression profiling of cultured cells from ASD probands thus serves as a window to underlying metabolic and signaling deficits that may be relevant to the pathobiology of autism.

ORGANISM(S): Homo sapiens

PROVIDER: GSE15451 | GEO | 2009/07/27

SECONDARY ACCESSION(S): PRJNA115947

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-15451 | biostudies-arrayexpress
| E-GEOD-21395 | biostudies-arrayexpress
2010-04-27 | GSE21395 | GEO
| E-GEOD-21086 | biostudies-arrayexpress
| E-GEOD-15402 | biostudies-arrayexpress
2023-02-05 | GSE212645 | GEO
2011-09-16 | GSE32136 | GEO
2021-03-21 | GSE99935 | GEO
| E-GEOD-65106 | biostudies-arrayexpress
| E-GEOD-32136 | biostudies-arrayexpress