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A functional link between Enhanced Disease Susceptibility 1 and GH3.12/avrPphB susceptible 3 in pattern-triggered immunity in plants

ABSTRACT: Pattern recognition receptors (PRRs) recognize microbial molecular patterns to enhance plant defenses through pattern-triggered immunity (PTI). In Arabidopsis, EFR and FLS2 provide PRRs for bacterial EF-Tu (elf18 epitope) and flagellin (flg22 epitope), respectively. Whether and how PRRs are diverged in defense signaling downstream of receptor complexes is unknown. Here we report that ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) is required for EFR- but not FLS2-mediated immunity against Pseudomonas syringae pv. tomato DC3000. Genome-wide profiling of elf18-induced transcriptional reprograming reveals defense-related genes that are deregulated in eds1 plants and rsw3, a dysfunctional glucosidase II± allele, which is specifically impaired in elf18-induced sustained defense activation. Negatively influenced elf18-inducible genes in eds1 and rsw3 plants include PBS3, encoding GH3 acyl adenylase. PBS3 also identifies a critical component in EFR-triggered immunity. Our findings point to a critical role for EDS1-mediated PBS3 induction in PTI and separate requirements between the EFR and FLS2 pathways.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE159523 | GEO | 2025/09/30

REPOSITORIES: GEO

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