Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sigma factor B (sigB) response to stress conditions (0.05% SDS and 5mM Diamide)

ABSTRACT: The numerous sigma factors present in Mycobacterium tuberculosis (MTB) are indicative of adaptability to different environmental conditions. In this report we describe the sigma factor B (sigB) regulon and the phenotypes of a MTB sigB mutant strain exposed to different stresses like SDS and Diamide. This experiment set compares expression profiles between H37Rv wild type and H37Rv sigB null mutant as well as under different stress conditions. Both H37Rv wild type and H37Rv sigB null mutants were treated with either 0.05% SDS or 5mM Diamide for 60 min and their expression profiles were compared with untreated wild type or mutant controls.

ORGANISM(S): Mycobacterium tuberculosis

PROVIDER: GSE15976 | GEO | 2009/06/23

SECONDARY ACCESSION(S): PRJNA115561

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Sigma factor B (sigB) response to stress conditions (0.05% SDS and 5mM Diamide)

Project description:The numerous sigma factors present in Mycobacterium tuberculosis (MTB) are indicative of adaptability to different environmental conditions. In this report we describe the sigma factor B (sigB) regulon and the phenotypes of a MTB sigB mutant strain exposed to different stresses like SDS and Diamide. This experiment set compares expression profiles between H37Rv wild type and H37Rv sigB null mutant as well as under different stress conditions. Both H37Rv wild type and H37Rv sigB null mutants were treated with either 0.05% SDS or 5mM Diamide for 60 min and their expression profiles were compared with untreated wild type or mutant controls. Biological Replicate

2010-06-25 | E-GEOD-15976 | biostudies-arrayexpress

The Mycobacterium tuberculosis ECF sigma Factor sE: role in global gene expression and survival in macrophages

Project description:In previously published work, we identified three Mycobacterium tuberculosis sigma (s) factor genes responding to heat shock (sigB, sigE and sigH ). Two of them (sigB and sigE ) also responded to SDS exposure. As these responses to stress suggested that the s factors encoded by these genes could be involved in pathogenicity, we are studying their role in physiology and virulence. In this work, we characterize a sigE mutant of M. tuberculosis H37Rv. The sigE mutant strain was more sensitive than the wild-type strain to heat shock, SDS and various oxidative stresses. It was also defective in the ability to grow inside both human and murine unactivated macrophages and was more sensitive than the wild-type strain to the killing activity of activated murine macrophages. Using microarray technology and quantitative reverse transcriptionÐpolymerase chain reaction (RTÐPCR), we started to define the sigmaE regulon of M. tuberculosis and its involvement in the global regulation of the stress induced by SDS. We showed the requirement for a functional sigE gene for full expression of sigB and for its induction after SDS exposure but not after heat shock. We also identified several genes that are no longer induced when sigmaE is absent. These genes encode proteins belonging to different classes including transcriptional regulators, enzymes involved in fatty acid degradation and classical heat shock proteins. Keywords: genetic modification design and comparative genome hybridization design

2007-08-03 | GSE8664 | GEO

Complex interactions of stress-associated regulons in an mprAB deletion mutant of Mycobacterium tuberculosis

Project description:Using a deletion mutant lacking portions of the response regulator, MprA, and the histidine kinase, MprB, we demonstrated by DNA microarrays that MprAB activates sigE and sigB under SDS stress and during logarithmic growth. Keywords: effects of 0.05% SDS on Mtb gene expression

2007-03-28 | GSE6750 | GEO

Transcription profiling of Mycobacterium tuberculosis wild type and sigE mutant strains after exposure to stress induced by SDS

2007-09-12 | E-TABM-313 | biostudies-arrayexpress

Mycobacterium tuberculosis

Project description:Sigma factor B (sigB) response to stress conditions (0.05% SDS and 5mM Diamide)

| PRJNA115561 | ENA

The Mycobacterium tuberculosis ECF sigma Factor sE: role in global gene expression and survival in macrophages

2007-08-02 | E-GEOD-8664 | biostudies-arrayexpress

Role of the extracytoplasmic-function sigma Factor sigmaH in Mycobacterium tuberculosis global gene expression

Project description:Like other bacterial species, Mycobacterium tuberculosis has multiple sigma (s) factors encoded in its genome. In previously published work, we and others have shown that mutations in some of these transcriptional activators render M. tuberculosis sensitive to various environmental stresses and, in some cases, cause attenuated virulence phenotypes. In this paper, we characterize a M. tuberculosis mutant lacking the ECF s factor sigma-H. This mutant was more sensitive than the wild type to heat shock and to various oxidative stresses, but did not show decreased ability to grow inside macrophages. Using quantitative reverse transcription-PCR and microarray technology, we have started to define the sigma-H regulon and its involvement in the global regulation of the response to heat shock and the thiol-specific oxidizing agent diamide. We identified 48 genes whose expression increased after exposure of M. tuberculosis to diamide; out of these, 39 were not induced in the sigH mutant, showing their direct or indirect dependence on sigma-H. Some of these genes encode proteins whose predicted function is related to thiol metabolism, such as thioredoxin, thioredoxin reductase and enzymes involved in cysteine and molybdopterine biosynthesis. Other genes under sigma-H control encode transcriptional regulators such as sigB, sigE, and sigH itself. Keywords: comparative genome hybridization design and genetic modification design

2007-08-04 | GSE8689 | GEO

Mycobacterium tuberculosis whiB4 mutant expression

2012-07-24 | GSE37840 | GEO

Transcription profiling of Mycobacterium tuberculosis wild type and sigH mutant strains after exposure to the thiol-specific oxidizing agent diamide

2007-09-12 | E-TABM-316 | biostudies-arrayexpress

Mycobacterium tuberculosis whiB4 mutant expression

Project description:Comparison of gene expression profile of the whiB4 mutant strain of Mycobacterium tuberculosis with the wild type Mycobacterium tuberculosis H37RV Mtb WhiB4 mutant mRNA was compared with the mRNA of wtMtb H37RV under aerobic conditons Aerbic conditions OD600 nm of 0.4, MtbWhiB4KO vs wtMtb, biological replicates: 3 wt Mtb H37RV and 3 MtbWhiB4 KO

2012-07-23 | E-GEOD-37840 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data