Dataset Information


Transcriptional analysis of the Neisseria gonorrhoeae Fur regulon

ABSTRACT: In Neisseria gonorrhoeae, Fur (ferric uptake regulator) protein regulates iron homeostasis gene expression through binding to conserved sequences in promoters of iron-responsive genes. We have expanded the gonococcal Fur regulon using a custom microarray to monitor iron-responsive gene expression throughout the growth curve combined with a genome-wide in silico analysis to predict Fur boxes (FB), and in vivo FuRTA assays to detect genes able to bind Fur. Keywords: time course: (1hr ,2hr, 3hr, 4hr) Overall design: The effect of iron on global gene expression was determined over a 4-h time period by comparing the RNA profile of the organism grown in iron-deplete conditions to growth in iron-replete conditions.

INSTRUMENT(S): OUHSC Dyer Neisseria gonorrhoeae 2K MPAUT1a520274F

SUBMITTER: Lydgia Jackson  

PROVIDER: GSE16352 | GEO | 2010-01-01



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Transcriptional and functional analysis of the Neisseria gonorrhoeae Fur regulon.

Jackson Lydgia A LA   Ducey Thomas F TF   Day Michael W MW   Zaitshik Jeremy B JB   Orvis Joshua J   Dyer David W DW  

Journal of bacteriology 20100101 1

To ensure survival in the host, bacteria have evolved strategies to acquire the essential element iron. In Neisseria gonorrhoeae, the ferric uptake regulator Fur regulates metabolism through transcriptional control of iron-responsive genes by binding conserved Fur box (FB) sequences in promoters during iron-replete growth. Our previous studies showed that Fur also controls the transcription of secondary regulators that may, in turn, control pathways important to pathogenesis, indicating an indir  ...[more]

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