Sequencing 5-formyluracil in genome DNA at single-base resolution

ABSTRACT: Albeit with low content, in genome DNA, the 5-formyluracil has been an important modification. The 5-formyluracil was found to be widely distributed amongst living bodies (prokaryotes and mammalian cells). Capable of binding to proteins to prevent the gene expressions, the 5-formyluracil could also induce mismatch in the DNA. In certain cancerous tissues, the content of 5-formyluracil was found to be higher than the normal tissues adjacent to the tumor. Nonetheless, the lack of a higher resolution mapping technique, has been hampering the studies of 5-formyluracil. Hence, we adopted the single-base resolution analysis method to profile 5-formyluracil at the genome scale. We analyzed the distribution of 5-formyluracil in the genome. This technique enabled us to have a better understanding of 5-formyluracil.

ORGANISM(S): Homo sapiens

PROVIDER: GSE164138 | GEO | 2022/12/01

REPOSITORIES: GEO