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Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing [scRNA-seq engrafted HSPCs]

ABSTRACT: Single cell RNA-seq analysis for WT and Fli-1 KO engrafted HSPCs (Lineage negative CD45.2+) was performed to assess changes in transcriptional output in HSPCs following Fli-1 deletion and Notch1 overexpression and to determine distribution of cells among the different hematopoietic populations.

ORGANISM(S): Mus musculus

PROVIDER: GSE171113 | GEO | 2024/04/01

REPOSITORIES: GEO

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Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing [Bulk ATAC-Seq]

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Transcriptional Activation of Regenerative Haematopoiesis via Niche Sensing

Project description:This SuperSeries is composed of the SubSeries listed below.

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Med12 and p300 chips in HSPCs

Project description:We characterized the genome wide occupancy of Med12 and p300 in mouse HSPCs. We also characterize p300 occupancy upon shRNA against control or Med12. ChIP-seq analysis of Med12 and/or p300 in untreated HSPCs

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Gene expression profile at the single cell level of CD45+ CD11b+ cells isolated from the brain and bone marrow of mice either untreated or conditioned and transplanted with mouse hematopoietic stem and progenitor cells (HSPCs) expanded in culture.

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