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RNA-seq of intestinal organoids after the first passage at day 5

ABSTRACT: The impact of MLL1 removal on intestinal organoids was evaluated in intestinal organoids from Mll1FC/+; RosaCreERT2/+ (control) and Mll1FC/FC; RosaCreERT2/+ (knockout) cultures after the first passage at day 5. Using 2x 100 bp reads, 50 million reads per sample with comparable unique mapped reads (74-81%) were obtained. To analyse differentially expressed genes, we applied DESeq2 analysis to the RNA-seq dataset. GO term analysis by DAVID at a false discovery rate (FDR) of 5% revealed that MLL1 was important for regulation of gene expression and differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE174050 | GEO | 2021/11/10

REPOSITORIES: GEO

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RNA-seq of intestinal organoids after the third passage at day 5

Project description:The impact of MLL1 removal on intestinal organoids was evaluated in intestinal organoids from Mll1FC/+; RosaCreERT2/+ (control) and Mll1FC/FC; RosaCreERT2/+ (knockout) cultures after the third passage at day 5. Using 2x 100 bp reads, 50 million reads per sample with comparable unique mapped reads (68-87%) were obtained. To analyse differentially expressed genes, we applied DESeq2 analysis to the RNA-seq dataset. GO term analysis by DAVID at a false discovery rate (FDR) of 5% revealed that MLL1 was important for regulation of gene expression and differentiation.

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2020-10-09 | GSE148394 | GEO

RNA-seq of Paneth cells

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RNA-seq of intestinal organoids after the first passage at day 5

Project description:RNA-seq of intestinal organoids after the first passage at day 5

| PRJNA728063 | ENA

First passage time distribution in heterogeneity controlled kinetics: going beyond the mean first passage time.

Project description: Not available

| S-EPMC4745057 | biostudies-other

Human small intestinal organoids grown with a specific protocol to enrich for Paneth cells were treated with an inhbitor (GSK-LSD1) for the epigenetic modulator LSD1.

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2020-11-16 | E-MTAB-7863 | biostudies-arrayexpress

Genome-wide identification and functional analysis of Apobec-1 mediated C-to-U RNA editing in mouse small intestine and liver

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RNA-seq of small intestinal organoids treated with an inhibitor for the epigenetic modifier LSD1

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2020-11-16 | E-MTAB-9077 | biostudies-arrayexpress

Cell-autonomous transformation of Lgr5-positive intestinal stem cells into gastric stem cells upon loss of transcription factor Cdx2

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