Genomics

Dataset Information

0

Small RNAs in budding yeast


ABSTRACT: RNAi, a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast, Saccharomyces cerevisiae. We report that RNAi is present in other budding-yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate siRNAs, which mostly correspond to transposable elements and Y´ subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y´ mRNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a novel class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.

ORGANISM(S): Vanderwaltozyma polyspora Saccharomyces cerevisiae Saccharomyces bayanus Candida albicans Naumovozyma castellii

PROVIDER: GSE17871 | GEO | 2009/09/10

SECONDARY ACCESSION(S): PRJNA123555

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-17871 | biostudies-arrayexpress
2009-09-10 | GSE17870 | GEO
| E-GEOD-17870 | biostudies-arrayexpress
2020-05-20 | GSE143548 | GEO
2019-08-21 | GSE129269 | GEO
2019-08-21 | GSE129232 | GEO
2019-08-21 | GSE90041 | GEO
2016-12-07 | GSE80474 | GEO
2018-07-31 | GSE75235 | GEO
| E-GEOD-52170 | biostudies-arrayexpress