Genomics

Dataset Information

21

DNA methylation in infant ALL


ABSTRACT: The aggressive MLL-rearranged leukemias are well-known for their unique gene-expression profiles. The goal of this study was to characterize the MLL-specific DNA methylation profiles in infant acute lymphoblastic leukemia (ALL). Genome-wide DNA methylation profiling was performed on primary infant ALL samples. The majority of infant ALL samples demonstrated severe DNA hypermethylation compared with normal pediatric bone marrows, which implies that targeting of DNA methylation may be an interesting option for future therapeutic strategies in MLL-rearranged infant ALL. Using ALL cell lines carrying the MLL translocation t(4;11) (SEMK2 and RS4;11) as a model for the patient cells, we demonstrated that the hypermethylated genes are sensitive to demethylation. Overall design: DNA methylation profiling was performed on t(4;11)-positive (n=16), t(11;19)-positive (n=15), t(9;11)-positive (n=6) and untranslocated (n=12) infant ALL samples compared with whole normal bone marrow samples (n=7). Cell lines SEMK2 and RS4;11 were profiled with and without the demethylating agent zebularine.

INSTRUMENT(S): Agilent-014791 Human CpG Island ChIP-on-Chip Microarray 244K (G4492A) (Feature Number version)

SUBMITTER: Dominique Stumpel  

PROVIDER: GSE18400 | GEO | 2009-11-23

SECONDARY ACCESSION(S): PRJNA118091

REPOSITORIES: GEO

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Publications

Specific promoter methylation identifies different subgroups of MLL-rearranged infant acute lymphoblastic leukemia, influences clinical outcome, and provides therapeutic options.

Stumpel Dominique J P M DJ   Schneider Pauline P   van Roon Eddy H J EH   Boer Judith M JM   de Lorenzo Paola P   Valsecchi Maria G MG   de Menezes Renee X RX   Pieters Rob R   Stam Ronald W RW  

Blood 20091023 27


MLL-rearranged infant acute lymphoblastic leukemia (ALL) remains the most aggressive type of childhood leukemia, displaying a unique gene expression profile. Here we hypothesized that this characteristic gene expression signature may have been established by potentially reversible epigenetic modifications. To test this hypothesis, we used differential methylation hybridization to explore the DNA methylation patterns underlying MLL-rearranged ALL in infants. The obtained results were correlated w  ...[more]

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