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Epigenetic reprogramming orchestrates transcriptional responses in shade avoidance syndrome of Arabidopsis

ABSTRACT: For shade-intolerant plants, changes in light quality indicative of competition from neighboring plants trigger shade avoidance syndrome (SAS). PYHTOCHROME-INTERACTING FACTOR 7 is the major transcriptional regulator of SAS in Arabidopsis. However, the epigenetic reprogramming under shade is poorly understood. To identify the histone chaperone ASF1 and HIRA function during SAS, we performed transcriptome deep sequencing (RNA-seq) to search for differentially expressed genes (DEGs) by comparing transcript levels between Col-0 and pif7-1, asf1ab, or hira-1 seedlings during white light and shade conditions. Our data shown that histone chaperone ASF1, through interacting with PIF7 and helping of HIRA, positively regulates shade-induced genes expression.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE189669 | GEO | 2023/01/30

REPOSITORIES: GEO

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PHYTOCHROME-INTERACTING FACTOR 7-mediated epigenetic reprogramming promotes the transcriptional response to shade in Arabidopsis

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Global transcriptome analysis identifies shade avoidance-related genes regulated by BBX24 in Arabidopsis thaliana

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PP2A mediates phosphorylation events during shade avoidance response in Arabidopsis

Project description:Many of shade-intolerant or sun-loving plants grown in close proximity experience a change in light quality and quantity, which leads to transcriptional reprogramming and consequently shade avoidance syndrome (SAS). Despite the importance of phosphorylation-dependent signaling for cellular physiology, the phosphorylation events during SAS are largely unknown. Here, we carried out a survey of shade-regulated protein phosphorylation events by a large-scale mass spectrometry-based quantitative phosphoproteomics approach. The activity of protein phosphatase 2A (PP2A) was specifically activated by shade treatment. Thereinto, shade de-phosphorlated a group of basic leucine zipper (bZIP) transcription factor by PP2A-b’ηθγζ. This study elucidates a previously unidentified mechanism by which the PP2A-bZIPs regulatory module integrates shade signal, and thus uncovers a phosphorylation strategy for the rapid response to changes of environment.

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A shade-induced long non-coding RNA promotes shade response through repressing the transcriptional induction of PHYTOCHROME A

Project description:A high-depth strand-specific RNA sequencing (ssRNA-seq) using 5-day-old white-light grown Col-0 and pif7-1 seedlings with additional 1 hr shade or white light treatment.

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