Transcriptomics,Genomics

Dataset Information

16

IL6 - IFNγ crosstalk experiment


ABSTRACT: This experiment tested whether pretreatment of human microvascular endothelial cells (HMECs) with interferon-gamma (IFNγ) for 2h affected the transcriptional response to 1h exposure to interleukin-6 (IL-6). Overall design: Human microvascular endothelial cells in culture; exposure to IFNγ 1 ng/ml for 3 h alone, with IFNγ 1 ng/ml for 2 h and then with IL6 50 U/ml for 1 h, with IL6 50 U/ml alone, or with neither. 4 replicates for each condition. RNA isolation, Illumina expression arrays. The supplementary file 'GSE19082_non-normalized_data.txt' contains non-normalized data for Samples GSM472434-GSM472449.

INSTRUMENT(S): Illumina humanRef-8 v2.0 expression beadchip

SUBMITTER: Branko Braam   

PROVIDER: GSE19082 | GEO | 2009-11-19

SECONDARY ACCESSION(S): PRJNA120493

REPOSITORIES: GEO

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