Transcriptomics,Genomics

Dataset Information

16

Genome-wide analysis of adipose tissue gene expression in twin-pairs discordant for physical activity for over 30 years


ABSTRACT: The high physical activity/high aerobic fitness phenotype predicts low morbidity and mortality more strongly than any other known biological risk factor. Inherited and/or acquired properties of skeletal muscle seem to be linked with aerobic fitness and other cardio-metabolic risk factors. However, physical activity-induced changes in muscle and fat tissue-related complex molecular networks and their links with cardio-metabolic disease risk are not comprehensively understood. In the present study, we show that among the physically active members of twin pairs, as compared to their inactive co-twins, gene expression in musculus vastus lateralis tissue samples is chronically up-regulated for the central pathways or genes related to energy metabolism, including oxidative phosphorylation, lipid metabolism and supportive metabolic pathways. In addition to aerobic fitness, upregulation of these pathways is associated in particular with high HDL cholesterol levels. In fat tissue we found physical activity-induced increases in the expression of polyunsaturated fatty acid metabolism and in branched chain amino acid degradation genes, increases which were associated with decreased ‘high-risk’ ectopic body fat and low glucose levels. Consistent with other findings, plasma lipidomics analyses showed up-regulation of the triacylglycerols containing the polyunsaturated fatty acids. The findings indicate the main pathways in skeletal muscle and fat tissue that mediate the chronic effects of physical activity on reduced cardio-metabolic disease risk, including the increase in aerobic fitness. Up-regulation of oxidative energy metabolism seems to be a common underlying mechanism. Overall design: Total RNA obtained from the biopsy samples of adipose tissue from two mono- and four dizygotic twin pairs that had been followed for their discordance for physical activity for 32 years. The supplementary file 'GSE20536_non-normalized.txt' contains non-normalized data for Samples GSM516113-GSM516124.

INSTRUMENT(S): Illumina HumanWG-6 v3.0 expression beadchip

SUBMITTER: Heikki Kainulainen   

PROVIDER: GSE20536 | GEO | 2010-07-31

SECONDARY ACCESSION(S): PRJNA125155

REPOSITORIES: GEO

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Publications

Differences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the members of physical activity discordant twin pairs.

Leskinen Tuija T   Rinnankoski-Tuikka Rita R   Rintala Mirva M   Seppänen-Laakso Tuulikki T   Pöllänen Eija E   Alen Markku M   Sipilä Sarianna S   Kaprio Jaakko J   Kovanen Vuokko V   Rahkila Paavo P   Oresic Matej M   Kainulainen Heikki H   Kujala Urho M UM  

PloS one 20100916 9


High physical activity/aerobic fitness predicts low morbidity and mortality. Our aim was to identify the most up-regulated gene sets related to long-term physical activity vs. inactivity in skeletal muscle and adipose tissues and to obtain further information about their link with cardio-metabolic risk factors. We studied ten same-sex twin pairs (age range 50-74 years) who had been discordant for leisure-time physical activity for 30 years. The examinations included biopsies from m. vastus later  ...[more]

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