Transcriptomics

Dataset Information

21

Effect of rosiglitazone on adipose gene expression in HSL knockout mice with different diet treatment


ABSTRACT: To test whether HSL is required for the supply of intrinsic ligands for PPARg for normal adipose differentiation, HSL-/- and wild type (WT) littermates were fed normal chow (NC) and high fat (HF) diets supplemented with or without rosiglitazone (200 mg/kg) for 16 weeks. The expression of diabetes related genes in the WAT of the animals was analyzed using Oligo GEArray Mouse Diabetes Microarrays (OMM-023, SABiosciences, Frederick, MD). Together with other biochemical and physiological data, our results suggest that one of the mechanisms by which HSL modulates adipose metabolism is by providing intrinsic ligands for PPARg. Overall design: To examine the changes in gene expression in WAT of WT and HSL-/- mice with rosiglitazone and diet treatment, RNA isolated from 4 animals of each group were used for studies of gene expression profiles using Oligo GEArray Mouse Diabetes Microarrays (OMM-023, SABiosciences, Frederick, MD). Data were analyzed using GEArray Expression Analysis Suite (SABiosciences, Frederick, MD) and SAM programs to comprehensively evaluate the differential gene expression of the various samples.

INSTRUMENT(S): The Oligo GEArray® Mouse Diabetes Microarray OMM-023

ORGANISM(S): Mus musculus  

SUBMITTER: Wen-Jun Shen  

PROVIDER: GSE20588 | GEO | 2012-03-18

SECONDARY ACCESSION(S): PRJNA125287

REPOSITORIES: GEO

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