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Integrative characterization of algal sulfated-fucan catabolism in marine heterotrophic bacteria

ABSTRACT: Sulfated fucans are key component of brown algal cell walls. These polysaccharides are particularly complex and structurally diverse, with multiple modifications (e.g. branches, sulfation, acetylation). A few bacterial enzymes involved in their biodegradation have been characterized but the catabolism of sulfated fucans remains largely uncharted. In Mariniflexile fucanivorans SW5, we discovered and studied a large Polysaccharide Utilization locus (PUL) specific for sulfated fucans. More than 20 enzymes have been characterized providing a model system for sulfated fucans utilization by marine heterotrophic bacteria. We further studied the transcriptomic responses of M. fucanivorans SW5 to sulfated fucan utilization demonstrating that this large PUL is indeed induced by sulfated fucans. Other gene clusters distantly localized in the genome are also strongly induced revealing a complex regulon. Notably genes involved into sulfite reduction are upregulated suggesting an adaptation to the abundance of sulfate ions released during the biodegradation of sulfated fucans.

ORGANISM(S): Mariniflexile fucanivorans

PROVIDER: GSE209534 | GEO | 2026/09/10

REPOSITORIES: GEO

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