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Transcriptomics of hypothalamic core areas suprachiasmatic nucleus and paraventricular nucleus during spontanous daily torpor arousal in the Djungarian hamster (Phodopus sungorus).

ABSTRACT: Transcriptome analyses were performed to further disentangle hypothalamic control of spontaneous daily torpor in the Djungarian Hamster (Phodopus sungorus) acclimated to winter-like short photoperiod (SP). Samples of hypothalamic core areas suprachiasmatic nucleus (SCN) and paraventricular nucleus (PVN) were taken about seven hours after lights on (ZT07). After a de novo assembly of transcriptomes and a mapping against RefSeq of the house mouse (Mus musculus), data from hamsters sampled with hypothermic (HT) core body temperature during torpor arousal on a torpor-day (TD) were compared with data from hamsters sampled with normothermic (NT) core body temperature on a torpor-free day (TFD). The data must be interpreted with respect to the metabolic and seasonal context, as described in the manuscript of the original research.

ORGANISM(S): Phodopus sungorus

PROVIDER: GSE214011 | GEO | 2026/06/24

REPOSITORIES: GEO

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Project description:Most temperate animals exhibit seasonal rhythms in reproductive physiology and behaviour. Gonadotropin-releasing hormone (GnRH) cells in the preoptic area (POA) and kisspeptin (Kiss) cells in the anteroventral periventricular (AvPv) nucleus are critical for timing photoperiod-induced changes in seasonal reproduction. In response to prolonged exposure to photoperiod cues, many rodents exhibit endogenously generated programmed changes in physiology that reflect a circannual timing mechanism. Here we used transcriptome sequencing coupled with high tissue sampling across a simulated circannual interval timer to characterise the molecular changes involved in the seasonal control of reproduction and body temperature. Adult male Djungarian hamsters were collected after exposure to short photoperiod on 4-week intervals from 4- to 32-weeks. The POA and AvPv transcriptomes from shot-photoperiod hamsters were compared to a long photoperiod reference group. Our analyses confirmed robust photoperiodic regulation of Kiss1 mRNA expression in the AvPv. Weighted gene co-expression network analyses (WGCNA) identified hundreds (POA: 211, AvPv: 415) of transcript that were associated with testes mass. Gene set enrichment analysis (GSEA) identified ‘gonadotropin secretion’ and ‘glial cell proliferation’ as the primary pathways associated with circannual interval timing. Vimentin had strong negative association with testes mass, indicating AvPv glial morphology increased during gonadal involution. In the POA, pathways associated with ‘regulation at synapse’ and ‘regulation of synaptic plasticity’ were highly enriched and transcripts positively associated with gonadal involution indicated that ribosomal plasticity and intracellular calcium signalling are key mechanisms involved in seasonal reproduction. WGCNA analyses identified 567 in the POA were significantly associated with surface body temperature. GSEA discovered thyrotrophin releasing hormone was significantly negatively associated with surface body temperature. Overall, the POA and AvPv transcriptome datasets provide the foundation to expand our understanding of the molecular representation of seasonal time in the mammalian hypothalamus.

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Transcriptomics of hypothalamic core areas suprachiasmatic nucleus and paraventricular nucleus during spontanous daily torpor arousal in the Djungarian hamster (Phodopus sungorus).

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