Transcriptomics

Dataset Information

34

Recipient cell nuclear factors are required for reprogramming by nuclear transfer


ABSTRACT: Reprogramming occurs after nuclear transfer into zygotes whose genome was removed in mitosis, but not after nuclear transfer into zygotes enucleated in interphase Egli et al. Development 2010 doi:10.1242/dev.046151 Overall design: Groups of 20 mouse embryos were used for the analysis. RNA amplification was done using Illumina total prep RNA amplification kit. Total of 21 arrays.

INSTRUMENT(S): Illumina mouseRef-8 v1.1 expression beadchip

ORGANISM(S): Mus musculus  

SUBMITTER: Dieter Egli  

PROVIDER: GSE21609 | GEO | 2010-05-01

SECONDARY ACCESSION(S): PRJNA126981

REPOSITORIES: GEO

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Publications

Recipient cell nuclear factors are required for reprogramming by nuclear transfer.

Egli Dieter D   Eggan Kevin K  

Development (Cambridge, England) 20100512 12


Nuclear transfer allows the reprogramming of somatic cells to totipotency. The cell cycle state of the donor and recipient cells, as well as their extent of differentiation, have each been cited as important determinants of reprogramming success. Here, we have used donor and recipient cells at various cell cycle and developmental stages to investigate the importance of these parameters. We found that many stages of the cell cycle were compatible with reprogramming as long as a sufficient supply  ...[more]

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