Transcriptomics

Dataset Information

32

Transcriptional failures arrest development after human nuclear transfer


ABSTRACT: Reprogramming occurs after nuclear transfer into zygotes whose genomes have been removed in mitosis, but not after nuclear transfer into zygotes enucleated in interphase. Our results suggest that there is a previously unappreciated barrier to successful human nuclear transfer, and that future studies should focus on the requirements for somatic genome activation. Overall design: 1-3 embryos were used for analysis. RNA amplification was done using two or three rounds of T7-mediated RNA amplification using the Illumina Total Prep RNA Amplification kit. Somatic cells 1-000 and 1-011 required only one round of RNA amplification because starting amounts of RNA were 100-500ng, while embryonic samples were amplified from single cells or embryos.

INSTRUMENT(S): Illumina HumanRef-8 v3.0 expression beadchip

ORGANISM(S): Homo sapiens  

SUBMITTER: Dieter Egli  

PROVIDER: GSE24631 | GEO | 2011-10-04

SECONDARY ACCESSION(S): PRJNA132461

REPOSITORIES: GEO

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