Transcriptomics,Genomics

Dataset Information

28

Asymmetric Reprogramming Capacity of Parental Pronuclei in Mouse Zygote


ABSTRACT: It has been demonstrated previously that the reprogramming factors are sequestered in the pronuclei of zygote after fertilization, as the enucleated zygotes at interphase cannot support the development of cloned embryos whereas the enucleated zygotes at M-phase can reprogram somatic cells to full pluripotency. However, it remains unknown whether the parental pronucleus, derived either from the sperm or oocyte, possesses the similar reprogramming ability. Here, we provide evidence demonstrating that the parental pronuclei are asymmetric in reprogramming and the reprogramming factors reside mainly in the male pronucleus. As a result, only the female pronucleus-depleted mouse zygotes enucleated at M-phase of mitosis can support the somatic cell reprogramming, the derivation of chromosome transfer embryonic stem (ctES) cells with full pluripotency and the full term development of cloned embryos. In striking contrast, the male pronucleus-depleted zygotes enucleated at M-phase of mitosis fail to support the pre-implantation development of somatic cell cloned embryos. Furthermore, we demonstrated that the distinct epigenetic reprogramming ability of the parental pronucleus might contribute directly to the developmental difference of somatic cloned embryos. Our study highlights the developmental asymmetry of parental pronuclei in reprogramming. Overall design: We compared the gene expression profile of ctES cells. Two biological repeats were included.

INSTRUMENT(S): [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]

SUBMITTER: wenqiang liu  

PROVIDER: GSE49148 | GEO | 2014-12-30

SECONDARY ACCESSION(S): PRJNA213147

REPOSITORIES: GEO

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Publications

Asymmetric reprogramming capacity of parental pronuclei in mouse zygotes.

Liu Wenqiang W   Yin Jiqing J   Kou Xiaochen X   Jiang Yonghua Y   Gao Haibo H   Zhao Yanhong Y   Huang Bo B   He Wenteng W   Wang Hong H   Han Zhiming Z   Gao Shaorong S  

Cell reports 20140313 6


It has been demonstrated that reprogramming factors are sequestered in the pronuclei of zygotes after fertilization, because zygotes enucleated at the M phase instead of interphase of the first mitosis can support the development of cloned embryos. However, the contribution of the parental pronucleus derived from either the sperm or the oocyte in reprogramming remains elusive. Here, we demonstrate that the parental pronuclei have asymmetric reprogramming capacities and that the reprogramming fac  ...[more]

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