SETD1A is required for intestinal homeostasis and prevention of DNA damage

ABSTRACT: The impact of SETD1A on the small intestine was evaluated in sorted intestinal stem, transit amplifying (TA) and Paneth cells from Setd1aFC/+; Lgr5-eGFP-CreERT2/+ (control) and Setd1aFC/FC; Lgr5-eGFP-CreERT2/+ (knockout) mice, 4 days after tamoxifen-induced mutagenesis. Using 100-base-pair reads, over 35 million reads per sample with comparable unique mapped reads were obtained. To analyze differentially expressed genes, we applied DESeq2 analysis to the RNA-seq dataset. Analysis by DAVID and GSEA at a false discovery rate (FDR) of 5% was conducted.The stem and TA cell transcriptome revealed that Setd1a knockout cells exhibited a decreased expression of several DNA damage repair genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE220632 | GEO | 2025/12/07

REPOSITORIES: GEO