Transcriptomics,Genomics

Dataset Information

41

In vitro and in vivo transcriptional profiling of Bacteroides thetaiotaomicron


ABSTRACT: Transcriptional profiling of Bacteroides thetaiotaomicron was performed on samples taken from (i) chemostat cultures (in vitro) and (ii) from the ceca of gnotobiotic monoassociated NMRI mice (in vivo). In vitro profiles were obtained from biological duplicate cultures taken at five timepoints from log to stationary phase in three types of media: (i) minimal media glucose (MM-G), (ii) minimal media maltotriose (MM-M), and (iii) rich media (TYG). In vivo profiling was performed on 12 mice, each colonized with B. thetaiotaomicron for ten days. The in vivo profiling was conducted in two separate experiments: (i) 6 male NMRI mice fed standard polysaccharide rich mouse chow and (ii) 3 male NMRI mice fed standard polysaccharide rich mouse chow and 3 male NMRI mice fed a simple sugar diet containing glucose and sucrose but deficient in polysaccharides. Keywords: other

INSTRUMENT(S): bthetaa511044F

SUBMITTER: Justin L. Sonnenburg  

PROVIDER: GSE2231 | GEO | 2005-03-30

SECONDARY ACCESSION(S): PRJNA91493

REPOSITORIES: GEO

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Publications

Glycan foraging in vivo by an intestine-adapted bacterial symbiont.

Sonnenburg Justin L JL   Xu Jian J   Leip Douglas D DD   Chen Chien-Huan CH   Westover Benjamin P BP   Weatherford Jeremy J   Buhler Jeremy D JD   Gordon Jeffrey I JI  

Science (New York, N.Y.) 20050301 5717


Germ-free mice were maintained on polysaccharide-rich or simple-sugar diets and colonized for 10 days with an organism also found in human guts, Bacteroides thetaiotaomicron, followed by whole-genome transcriptional profiling of bacteria and mass spectrometry of cecal glycans. We found that these bacteria assembled on food particles and mucus, selectively induced outer-membrane polysaccharide-binding proteins and glycoside hydrolases, prioritized the consumption of liberated hexose sugars, and r  ...[more]

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