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Lysine-specific histone demethylase 1A (KDM1A/LSD1) inhibition attenuates DNA double strand break repair and augments efficacy of temozolomide in glioblastoma

ABSTRACT: Patient-derived primary GSCs (GSC082209) were established from discarded specimens obtained from glioblastoma (GBM) patients undergoing surgery at UT Health San Antonio. We examined global transcriptional changes by RNA-sequencing using patient derived glioma stem cells (GSCs) that are transduced with KDM1A gRNA to knockout KDM1A. For KDM1A knockout, Scramble and KDM1A-specific gRNA were transduced into GSCs possessing TET-inducible Cas9. Cas9 stimulation was done by treating GSCs with 50 ng/mL doxycycline for 14 days, after which cells were validated for KDM1A knockout.The RNA was isolated and utilized for RNA-seq analysis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE223335 | GEO | 2026/01/10

REPOSITORIES: GEO

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