Transcriptomics

Dataset Information

30

Gene expression profiling of 3T3 fibroblast cells cultured with degradable metallic materials


ABSTRACT: Metals have been used for coronary artery stent materials in order to prevent stenosis caused by atherosclerosis. Degradable metallic materials (DMMs) are considered to be useful to avoid in-stent restenosis and late thrombosis. A new DMM, Fe-35Mn alloy, was fabricated through powder metallurgy in order to satisfy the ideal criteria of cardiovascular stent made of DMM. Since in-stent restenosis is mediated by the extracellular matrix production, which is mostly regulated by fibroblasts, the gene expression profile of 3T3 fibroblasts in the presence of Fe-35Mn alloy was then investigated. The mechanism of cellular responses in the presence of DMM is then expected to be clearly described through the gene profiling experiment. Overall design: 3T3 fibroblast cells derived from mouse (Mus musculus) embryo (BALB/3T3 clone A31, ATTC) were put in the culture and treated with iron, manganese, and Fe-35Mn alloy for 24 hour in parallel and followed by the RNA extraction. Six technical replicates were included for treated cells as well as control cells.

INSTRUMENT(S): Illumina MouseWG-6 v2.0 expression beadchip

ORGANISM(S): Mus musculus  

SUBMITTER: Jacques Couet  

PROVIDER: GSE22794 | GEO | 2010-07-24

SECONDARY ACCESSION(S): PRJNA127817

REPOSITORIES: GEO

Similar Datasets

2010-07-24 | E-GEOD-22794 | ArrayExpress
| PRJNA127817 | ENA
2014-01-08 | E-GEOD-53857 | ArrayExpress
| GSE85391 | GEO
2008-06-15 | E-GEOD-6794 | ArrayExpress
2007-01-23 | GSE6794 | GEO
2015-05-28 | E-GEOD-69320 | ArrayExpress
2016-11-10 | E-MTAB-5113 | ArrayExpress
2010-03-01 | GSE19136 | GEO
2010-03-07 | E-GEOD-19136 | ArrayExpress