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Analysing environmentally induced genome-wide frameshift in S. cerevisiae and bacteria using 5PSeq

ABSTRACT: In this study, we examine the impact of poor nutrient conditions on -1 ribosome frameshifting and its effect on mRNA degradation.To explore this process, we utilized HT-5PSeq (Zhang & Pelechano, 2021, PMID:35474692) to measure the ribosome footprint of co-translationally degraded mRNAs in S.cerevisiae under different stresses in YPD and CSM (poor nutrients). To show the mechanism is conserved in bacteria, we performed HT-5PSeq in B.subtilis upon starvation .Our goal with HT-5PSeq is to identify gene- and codon-specific features that contribute to genome-wide frameshifting events.

ORGANISM(S): Bacillus subtilis Saccharomyces cerevisiae

PROVIDER: GSE230202 | GEO | 2025/05/14

REPOSITORIES: GEO

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Analysing environmentally induced genome-wide frameshift in S. cerevisiae using ribosome profiling

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Analysing environmentally induced genome-wide frameshift in S. cerevisiae and bacteria using 5PSeq

Project description:Analysing environmentally induced genome-wide frameshift in S. cerevisiae and bacteria using 5PSeq

| PRJNA957826 | ENA

Analysing environmentally induced genome-wide frameshift

Project description:This SuperSeries is composed of the SubSeries listed below.

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