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Analysing environmentally induced genome-wide frameshift in S. cerevisiae and bacteria using 5PSeq

ABSTRACT: Analysing environmentally induced genome-wide frameshift in S. cerevisiae and bacteria using 5PSeq

PROVIDER: PRJNA957826 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR24233900.fastq.gz	Fastqsanger.gz
	SRR24233901.fastq.gz	Fastqsanger.gz
	SRR24233852.fastq.gz	Fastqsanger.gz
	SRR24233853.fastq.gz	Fastqsanger.gz
	SRR24233854.fastq.gz	Fastqsanger.gz

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Analysing environmentally induced genome-wide frameshift

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Project description:In this study, we examine the impact of poor nutrient conditions on -1 ribosome frameshifting and its effect on mRNA degradation. To measure the RNA degradation, we utilized SLAM-seq (Herzog, et.al, 2017, PMID: 28945705) in S.cerevisiae in wildtype and NMD mutant (upf1Δ). Our goal with SLAM-seq is to quantify the fraction of the transcriptome that is directly degraded as a result of environmentally induced ribosome frameshifts through an NMD-dependent mechanism.

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