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Analysing environmentally induced genome-wide frameshift in S. cerevisiae using SLAM-Seq

ABSTRACT: Analysing environmentally induced genome-wide frameshift in S. cerevisiae using SLAM-Seq

PROVIDER: PRJNA957829 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24233943.fastq.gz	Fastqsanger.gz
	SRR24233944.fastq.gz	Fastqsanger.gz
	SRR24233945.fastq.gz	Fastqsanger.gz
	SRR24233946.fastq.gz	Fastqsanger.gz
	SRR24233947.fastq.gz	Fastqsanger.gz

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Analysing environmentally induced genome-wide frameshift

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Analysing environmentally induced genome-wide frameshift in S. cerevisiae using SLAM-Seq

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Analysing environmentally induced genome-wide frameshift

Project description:This SuperSeries is composed of the SubSeries listed below.

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Analysing environmentally induced genome-wide frameshift in S. cerevisiae using ribosome profiling

Project description:In this study, we examine the impact of poor nutrient conditions on -1 ribosome frameshifting and its effect on mRNA degradation. To explore this process, we utilized Ribosome profiling (Ingolia, et.al 2009, PMID: 19213877), in S.cerevisiae in low nutrients condition. Our goal with Ribosome profiling was to identify gene- and codon-specific features that contribute to genome-wide frameshifting events.

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Project description:In this study, we examine the impact of poor nutrient conditions on -1 ribosome frameshifting and its effect on mRNA degradation.To explore this process, we utilized HT-5PSeq (Zhang & Pelechano, 2021, PMID:35474692) to measure the ribosome footprint of co-translationally degraded mRNAs in S.cerevisiae under different stresses in YPD and CSM (poor nutrients). To show the mechanism is conserved in bacteria, we performed HT-5PSeq in B.subtilis upon starvation .Our goal with HT-5PSeq is to identify gene- and codon-specific features that contribute to genome-wide frameshifting events.

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