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Dataset Information

46

Genome-wide analysis of insulin-like growth factor 1 receptor (IGF1R) binding sites in DFB cells


ABSTRACT: The insulin-like growth factor 1 receptor (IGF-1R) plays crucial roles in developmental and cancer biology. Most of its biological effects have been ascribed to its tyrosine kinase activity. We report that IGF-1 promotes the modification of IGF-1R by small ubiquitin-like modifier protein-1 (SUMO-1) and its translocation to the nucleus. Nuclear IGF-1R associated with enhancer-like elements and increased transcription in reporter assays. We used ChIP-seq to examine the interaction of IGF-1R with DNA on a genome-wide scale. Analysis of the data set resulted in 568 candidate peaks, that is, statistically significant IGF-1R-enriched regions. The IGF-1R-enriched regions were divided into five classes on the basis of their location relative to known genes. Most of the IGF-1R-interacting sites (80%) were located distal from any annotated gene (intergenic), 6.3% were located in introns, 6.3% in exons, 3.4% were <20 kb upstream of an annotated transcript start site (5'UTR + 20 kb upstream), and 3.6% were <20 kb downstream of an annotated transcript end site (3'UTR + 20 kb downstream). Overall design: Analysis of the genomic interaction of IGF1R in DFB cells

INSTRUMENT(S): Illumina Genome Analyzer (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Ali Tofigh  

PROVIDER: GSE23792 | GEO | 2010-08-25

SECONDARY ACCESSION(S): PRJNA130715

REPOSITORIES: GEO

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