Lactobacillus plantarum IMDO 130201, a wheat sourdough isolate, adapts to growth in wheat sourdough simulation medium at different pH values through differential gene expression

ABSTRACT: Sourdough is a very competitive and challenging environment for microorganisms. Usually, a stable microbiota composed of lactic acid bacteria (LAB) and yeasts comes to dominate this ecosystem. Although rich in carbohydrates, thus providing an ideal environment to grow, the low pH presents a particular challenge. The nature of the adaptation to this low pH was investigated for Lactobacillus plantarum IMDO 130201, an isolate from a laboratory wheat sourdough fermentation. Batch fermentations were carried out in wheat sourdough simulation medium, and total RNA was isolated from mid-exponential growth phase cultures, followed by differential gene expression analysis using a LAB functional gene microarray. At low pH values, an increased expression of genes involved in peptide and amino acid metabolism was observed as well as of genes involved in plantaricin production and lipoteichoic acid synthesis. The results highlight cellular mechanisms that allow L. plantarum to function at a low environmental pH.

ORGANISM(S): Lacticaseibacillus casei Companilactobacillus farciminis Limosilactobacillus panis Weissella confusa Lactobacillus helveticus Latilactobacillus curvatus Lentilactobacillus hilgardii Lactococcus lactis Enterococcus mundtii Lentilactobacillus buchneri Streptococcus thermophilus Brevibacterium linens Lactobacillus gasseri Pediococcus pentosaceus Limosilactobacillus pontis Lactobacillus crispatus Lactobacillus acidophilus Fructilactobacillus sanfranciscensis Latilactobacillus sakei Companilactobacillus alimentarius Oenococcus oeni Lactiplantibacillus pentosus Enterococcus faecium Lactobacillus delbrueckii Bifidobacterium Lacticaseibacillus paracasei Lentilactobacillus parabuchneri Fructilactobacillus fructivorans Companilactobacillus mindensis Leuconostoc mesenteroides Lactiplantibacillus plantarum Companilactobacillus paralimentarius Lacticaseibacillus rhamnosus Furfurilactobacillus rossiae Limosilactobacillus fermentum Enterococcus faecalis Leuconostoc pseudomesenteroides Mammaliicoccus lentus Enterococcus casseliflavus Leuconostoc citreum Lactiplantibacillus paraplantarum Lactobacillus johnsonii Pediococcus acidilactici Lactobacillus amylovorus Staphylococcus aureus Enterococcus hirae Limosilactobacillus reuteri Levilactobacillus brevis Limosilactobacillus frumenti

PROVIDER: GSE23945 | GEO | 2011/08/01

SECONDARY ACCESSION(S): PRJNA130407

REPOSITORIES: GEO