Project description:Jurkat T cells (in triplicate per treatment group) were left untreated in culture, infected with VSV-G-pseudotyped HIV-based vector (which transduces Tat and eGFP) or treated with TNFalpha. Keywords: parallel sample
Project description:To explore the function of ERRalpha in viral infection, we compared the cDNA expression profile of ERRalpha knockdown 293T cells and control 293T cells. The cells infected with VSV for 12 h or left uninfected were collected and RNA was extracted by Trizol.
Project description:Micorarray analysis was performed on RNA samples from hippocampal cultures infected with either Ad-aCaN or Ad-LacZ or uninfected. Each sample was applied to its own GeneChip (Rat RG-U34A; n=7-9 chips/group). Chips were then processed and scanned using Agilent Affymetrix GeneArray Scanner. MAS5 was used to determine signal intensity and presence/absence calls for the data. Keywords = bioinformatics Keywords = gene expression Keywords = calcineurin Keywords = calcium Keywords = Alzheimer's Keywords = inflamation Keywords = Adenovirus Keywords = astrocytes Keywords = microarray Keywords: parallel sample
Project description:Biologic functions involved in innate immune response of macrophages rely on the precise regulation of kinds of immune molecular. In the virus infection procession, the macrophages are activated following a tightly controlled genetic programme where specific sets of genes are up-regulated or down-regulated. We used microarrays to detail the global programme of gene expression underlying VSV infection and identified distinct classes of up-regulated and down-regulated genes during this process. Mouse peritoneal macrophages were selected with/without VSV infection for 8 hours for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain expression profiles. We selected macrophages according to VSV infection at two time-points: uninfected macrophage(control) and VSV infected for 8 hour macrophages(VSV).
Project description:This study examines the genome-wide binding profile of the transcription factor IRF1 in bone marrow-derived macrophages (BMDMs) infected with vesicular stomatitis virus (VSV) compared to uninfected controls. BMDMs were differentiated from mouse bone marrow cells via M-CSF stimulation and then infected with VSV (MOI = 1) for 4 hours or mock-treated. Chromatin immunoprecipitation (ChIP) was performed using an anti-IRF1 antibody, followed by next-generation sequencing (Illumina). Data analysis included alignment to the mm10 genome, peak calling, and coverage normalization to identify IRF1 binding sites under viral infection. These results provide insights into IRF1's regulatory role in antiviral immune responses.
Project description:DDX46 is identified to be required at the early step of pre-spliceosome assembly,but the potential roles of DDX46 in RNA editing and whether DDX46 could regulate antiviral innate immunity by editing antiviral transcripts in the nucleus remain elusive. iCLIP-Seq analyses of DDX46-bound RNAs from uninfected and VSV-infected RAW264.7 cells
Project description:Human 293T cells engineered to express the TVA receptor (see Mitchell et al., PLoS) infected with an ASLV vector. RNA isolated 48 hours later. ***PLEASE NOTE*** Series entries GSE1408 & GSE1409 no longer exist. All data discussed in the author's PLoS publication (PubMed ID 15314653) is available under GSE1407 & GSE1410. ***PLEASE NOTE*** Keywords: repeat sample