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LXR agonism enhances T cell activation upon crosspresentation by LXR-DKO BMDCs.

ABSTRACT: Here, we analyze transcriptional profiles of OT-I CD8+ T cells co-cultured with LXR-/- bone marrow-derived dendritic cells pulsed with the SIINFEKL peptide in the absence or presence of the synthetic LXR agonist RGX-104.

ORGANISM(S): Mus musculus

PROVIDER: GSE249710 | GEO | 2025/12/30

REPOSITORIES: GEO

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LXR agonism enhances T cell activation upon crosspresentation.

Project description:Here, we analyze transcriptional profiles of OT-I CD8+ T cells co-cultured with bone marrow-derived dendritic cells pulsed with the SIINFEKL peptide in the absence of presence of the synthetic LXR agonist RGX-104.

2025-12-29 | GSE249709 | GEO

LXR agonism promotes T cell effector chromatin landscapes upon crosspriming by dendritic cells.

Project description:Here, we assess chromatin accessibility profiles of OT-I CD8+ T cells upon co-culture with antigen-pulsed BMDCs in the presence or absence of the synthetic LXR agonist RGX-104.

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LXR agonism elicits a pre-effector state in naïve CD8+ T cells [RNA-seq]

Project description:Here, we analyze transcriptional profiles of naïve CD8+ T cells with versus without exposure to the synthetic LXR agonist RGX-104 in the absence of TCR stimulation.

2025-12-30 | GSE303316 | GEO

LXR agonism elicits a pre-effector state in naïve CD8+ T cells [ATAC-seq]

Project description:Here, we assess chromatin accessibility profiles of naïve CD8+ T cells after 24 hours of exposure to either the LXR agonist RGX-104 or DMSO in the absence of TCR stimulation.

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scRNA-seq and TCR-seq of T cells and APCs in tumor-draining lymph nodes with versus without pharmacological LXR activation.

Project description:Here we assessed the impact on the transcriptomes of T cells and APCs in tumor-draining lymph nodes from mice administered control chow versus chow containing the synthetic LXR agonist RGX-104. In addition, TCR-seq was performed to assess the impact on clonal expansion of T cells upon LXR activation.

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CD8 T cell transcriptional changes induced by MDSCs during TCR stimulation

Project description:Single cell RNA-seq analyiss of FACS enriched OT-I CD8+ T cells following co-culture with SIINFEKL-pulsed bone marrow-derived DCs and bone marrow-derived myeloid-derived suppressor cells (T cell:DC:MDSC = 1:0.2:2)

2021-05-26 | GSE145396 | GEO

Impact of LXR activation on alternatively activated human macrophages

Project description:To investigate how LXR activation potentiates expression of Th2 cytokine-dependent genes in primary human macrophages, we pulsed macrophages with synthetic LXR ligand T0901317 then polarized cells to alternatively activated macrophages with IL-4 or IL-13.

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Immunomodulatory-Photodynamic Nanostimulators for Invoking Pyroptosis to Augment Tumor Immunotherapy

Project description:Acidic tumor microenvironment (TME)-evoked MRC nanoparticles (MRC NPs) co-delivering immune agonist RGX-104 and photosensitizer chlorine e6 (Ce6) are reported for pyroptosis-mediated immunotherapy. RGX-104 remodels TME by transcriptional activation of ApoE to regress myeloid-derived suppressor cells’ (MDSCs) activity, which neatly creates foreshadowing for intensifying pyroptosis. Considering Ce6-triggered photodynamic therapy (PDT) can strengthen oxidative stress and organelles destruction to increase immunogenicity, immunomodulatory-photodynamic MRC nanodrugs will implement an aforementioned two-pronged strategy to enhance gasdermin E (GSDME)-dependent pyroptosis. RNA-seq analysis of MRC at the cellular level is introduced to first elucidate the intimate relationship between RGX-104 acting on LXR/ApoE axis and pyroptosis, where RGX-104 provides the prerequisite for pyroptosis participating in antitumor therapy. Briefly, MRC with favorable biocompatibility tackles the obstacle of hydrophobic drugs delivery, and becomes a powerful pyroptosis inducer to reinforce immune efficacy. MRC-elicited pyroptosis in combination with anti-PD-1 blockade therapy boosts immune response in solid tumors, successfully arresting invasive metastasis and extending survival based on remarkable antitumor immunity. MRC may initiate a new window for immuno-photo pyroptosis stimulators augmenting pyroptosis-based immunotherapy. Owing to the transcriptome sequencing on 4T1 cells exposed to various treatment, pyroptosis mechanism of MRC was thoroughly analyzed via comparing the expression discrepancy among PBS, MRC, and MRC+L groups.

2023-08-13 | GSE240328 | GEO

Effect of loss of PKC theta and p50+cRel on gene expression post T-cell stimulation

Project description:OT-1 Transgenic CD8 T-cells were isolated from spleens of WT, PKCθ theta KO, and p50 cRel DKO mice. The T-cells were either cultured with non-pulsed DC (WT only and signified as "WT - UN") or with BMDCs pulsed with the OVA peptide SIINFEKL (N4) (WT, PKCθ theta KO, and p50 cRel DKO and signified as 'genotype - N4') at a ratio of 1:10 (DC:T-cell) for 18 hours. DCs then were depleted from the culture and RNA was made from the T-cells to measure gene expression at the early / late stage of T-cell activation

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OT-I T cell activation in Lrrc8af/f and Lrrc8af/f Cd4-Cre;OT-I mice

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