Genomics

Dataset Information

60

[E-TABM-828] ChIP-Seq in human MCF7 and HEPG2 cells


ABSTRACT: ChIP-Seq study in human MCF7 and HEPG2 cells using antibodies against CTCF (Millipore, 07-729), STAG1 (abcam, ab4457), RAD21 (abcam, ab992), ERa (santa cruz, sc-543), CEBPa (santa cruz, sc-9314) ArrayExpress Release Date: 2010-05-12 Publication Title: A CTCF-independent role for cohesin in tissue-specific transcription Publication Author List: Dominic Schmidt, Petra C. Schwalie, Caryn S. Ross-Innes, Antoni Hurtado, Gordon D. Brown, Jason S. Carroll, Paul Flicek and Duncan T. Odom Person Roles: submitter Person Last Name: Schwalie Person First Name: Petra Catalina Person Email: schwalie@ebi.ac.uk Person Address: Person Affiliation: EBI Overall design: Experimental Design: binding_site_identification_design Experimental Design: high_throughput_sequencing_design Experimental Design: ChIP-seq

INSTRUMENT(S): Illumina Genome Analyzer (Homo sapiens)

SUBMITTER: ArrayExpress EBI  

PROVIDER: GSE25021 | GEO | 2011-02-05

SECONDARY ACCESSION(S): PRJNA134393

REPOSITORIES: GEO

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Publications

A CTCF-independent role for cohesin in tissue-specific transcription.

Schmidt Dominic D   Schwalie Petra C PC   Ross-Innes Caryn S CS   Hurtado Antoni A   Brown Gordon D GD   Carroll Jason S JS   Flicek Paul P   Odom Duncan T DT  

Genome research 20100310 5


The cohesin protein complex holds sister chromatids in dividing cells together and is essential for chromosome segregation. Recently, cohesin has been implicated in mediating transcriptional insulation, via its interactions with CTCF. Here, we show in different cell types that cohesin functionally behaves as a tissue-specific transcriptional regulator, independent of CTCF binding. By performing matched genome-wide binding assays (ChIP-seq) in human breast cancer cells (MCF-7), we discovered thou  ...[more]

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