Project description:To examine the response of Candida glabrata cells to iron-depleted and iron-repleted environmental conditions, transcriptional profiling analysis was carried out on wild-type and Cghog1∆ cells grown either in presence of BPS or ferric chloride. Genes involved in iron transport and homeostasis, oxidative phosphorylation, amino acid metabolic process and chromatin silencing were found to be differentially regulated. Overall design: Agilent one-color experiment,Organism: Candida glabrata, Agilent Platform Custom Candida glabrata 8x15k designed by Genotypic Technology Private Limited.(AMADID: 49117), Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)
Project description:Whole genome microarrays were used to generate the transcriptional profile of Candida parapsilosis in low iron conditions RNA was isolated from CLIB214 grown in SD medium and in SD medium supplemented with 200 μM BPS and labeled with Cy3 or Cy5. Seven independent biological replicates were compared. Three dye swaps were perfomed so that four out of seven samples grown in SD culture were labeled with Cy3, and 3 were labeled with Cy5.
Project description:A Candida glabrata wild type strain (HTL, as described in Schwartzmuller et al., PLoS Pathog. 2014 Jun 19;10(6):e1004211) was submitted to various stress conditions (iron excess, salt excess, cadmium treatment and iron starvation (BPS treatment). The cells were collected 20 and 40 minutes after the beginning of treatments and their transcriptomes were compared to those of mock treated cells.
Project description:The mammalian gastrointestinal tract and the bloodstream are highly disparate biological niches, and yet certain commensal-pathogenic microorganisms are able to thrive in both environments. Here, we report the evolution of a unique transcription circuit in the yeast, Candida albicans, which determines its fitness in both host niches. Our comprehensive analysis of the DNA-binding proteins that regulate iron uptake by this organism suggests the evolutionary intercalation of a transcriptional activator called Sef1 between two broadly conserved transcriptional repressors, Sfu1 and Hap43. The Sef1 activator of iron uptake genes promotes virulence in a mouse model of bloodstream infection, whereas the Sfu1 repressor is dispensable for virulence but promotes gastrointestinal commensalism. We propose that the ability to alternate between genetic programs conferring resistance to iron depletion in the bloodstream versus iron toxicity in the gut may be a fundamental attribute of gastrointestinal commensal-pathogens. Overall design: The Wild type strain (SN250) was grown in either YEPD or YEPD supplemented with iron chelator bathophenanthroline disulfonate (BPS); The sfu1Δ (SN515) mutant was grown in YEPD medium; The sef1Δ(SN330) or hap43Δ (SN694) mutant was grown YEPD supplemented with iron chelator bathophenanthroline disulfonate (BPS). 5-6 biological replicates were performed per strain per condition. RNA was prepared from these strains and samples were hybridized on custom Agilent C. albicans ORF arrays (15,000 spots/array, 70-mer probes).
Project description:Heme Deficient or Wild Type (normal heme) for 6 hrs with iron chelation by BPS. Single Experiment with Probe Reversal 1. GSM78518 (Cy3) vs GSM78519 (Cy5) 2. GSM78520 (Cy3) vs GSM78521 (Cy5)
Project description:Transcriptional time series of Candida glabrata under iron starvation (SD medium without Fe). Wild type and deletion mutants of the iron-related transcription factors Aft1 and Sef1, as well as of the iron uptake transporter Ftr1 as a positive control. Overall design: Common reference design, reference is log-phase wild type in YPD complex medium
Project description:Transcriptome profiling to identify Cap2/Hap43 regulons in the human fungal pathogen Candida albicans: Wild type vs. cap2D grown in iron-depleted medium Overall design: Two-condition experiment, WT vs. cap2D cells. Biological replicates: Wild-type and cap2D, independently grown in iron-depleted medium and harvested. One replicate per array. Hybridization was repeated (technical replicate) for each biological replicate.
Project description:Transcriptome profiling to identify Cap2/Hap43 regulons in the human fungal pathogen Candida albicans: Wild type vs. cap2D grown in iron-depleted medium Two-condition experiment, WT vs. cap2D cells. Biological replicates: Wild-type and cap2D, independently grown in iron-depleted medium and harvested. One replicate per array. Hybridization was repeated (technical replicate) for each biological replicate.