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Multi-omics of Sex-Based Differences in Nephrotoxic Serum Nephritis [scRNA-seq]

ABSTRACT: Sex-based differences have been observed in the incidence and clinical course of acute kidney injury as well as chronic kidney disease. Compared to males, female patients are more protected from kidney injury and disease progression. Prior studies have identified sexual dimorphism in kidney development and ischemic response, but the mechanisms by which inflammatory stimuli contribute to kidney sex based differences have not been fully elucidated. To examine these differences, we induced nephrotoxic serum (NTS) nephritis in female and male C57BL/6 mice of either 8-12 weeks or 18 months of age. Kidneys were harvested on Day 10. Nuclei were isolated and multiomics sequencing (matched snRNA-seq and snATAC-seq) was performed on individual nuclei.

ORGANISM(S): Mus musculus

PROVIDER: GSE262595 | GEO | 2025/10/31

REPOSITORIES: GEO

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Multi-omics of Sex-Based Differences in Nephrotoxic Serum Nephritis [scATAC-seq]

Project description:Sex-based differences have been observed in the incidence and clinical course of acute kidney injury as well as chronic kidney disease. Compared to males, female patients are more protected from kidney injury and disease progression. Prior studies have identified sexual dimorphism in kidney development and ischemic response, but the mechanisms by which inflammatory stimuli contribute to kidney sex based differences have not been fully elucidated. To examine these differences, we induced nephrotoxic serum (NTS) nephritis in female and male C57BL/6 mice of either 8-12 weeks or 18 months of age. Kidneys were harvested on Day 10. Nuclei were isolated and multiomics sequencing (matched snRNA-seq and snATAC-seq) was performed on individual nuclei.

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Multi-omics of Sex-Based Differences in Nephrotoxic Serum Nephritis [scRNA-seq]

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