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Systematic discovery of subcellular RNA patterns in the gut epithelium

ABSTRACT: Subcellular RNA localization is crucial for the spatio-temporal control of protein synthesis and underlies key processes during development, homeostasis and disease. In epithelial cells, RNA can localize asymmetrically along the apico-basal axis. Yet, we ignore the localization of most transcripts as well as the diversity of patterns that they adopt. Here, we utilized proximity labeling (APEX-seq) and spatial transcriptomics (MERFISH) to map subcellular transcript localization in intestinal organoids and adult mice tissue. Many transcripts presented localization bias, often localizing in granular structures. We described the intrinsic and environmental factors that influence the formation of these patterns. We identified translation-dependent and -independent localization patterns and pintointed the role of 3’ UTRs and RNA-binding proteins. This subcellular RNA atlas can be referenced to extract new biological insights and provide hypothesis-free observation on spatial transcriptomics.

ORGANISM(S): Mus musculus

PROVIDER: GSE267163 | GEO | 2025/09/05

REPOSITORIES: GEO

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