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Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [proximal sciatic nerve]

ABSTRACT: We applied Solexa sequencing technology to identify rat microRNA genes in proximal sciatic nerve following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 93 novel miRNAs in rats were discovered and identified, of which 42 novel miRNAs were first reported in proximal sciatic nerve of rat and 51 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration. Keywords: Small RNA sequencing

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE26753 | GEO | 2012/05/06

SECONDARY ACCESSION(S): PRJNA136341

REPOSITORIES: GEO

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Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [proximal sciatic nerve]

Project description:We applied Solexa sequencing technology to identify rat microRNA genes in proximal sciatic nerve following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 93 novel miRNAs in rats were discovered and identified, of which 42 novel miRNAs were first reported in proximal sciatic nerve of rat and 51 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration. Keywords: Small RNA sequencing 18-30 nt small RNAs from proximal sciatic nerve of 30 Thirty Sprague-Dawley (SD) rats were sequenced at one Solexa lane

2012-05-06 | E-GEOD-26753 | biostudies-arrayexpress

Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [dorsal root ganglia]

Project description:We applied Solexa sequencing technology to identify rat microRNA genes in dorsal root ganglia (DRGs) following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 114 novel miRNAs in rats were discovered and identified, of which 52 novel miRNAs were first reported in rat DRGs and 62 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration.

2012-05-06 | GSE25863 | GEO

Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [dorsal root ganglia]

2012-05-06 | E-GEOD-25863 | biostudies-arrayexpress

Gene expression signatures following nerve injury in a rat model (0h-9h)

Project description:We used microarrays to distinguish the gene expression differences among different time points after injury. We generated proximal sciatic nerve (SN) tissues (0.5cm) at 0h, 0.5h, 1h, 3h, 6h and 9h after sciatic nerve resection.

2012-12-20 | E-GEOD-33175 | biostudies-arrayexpress

Gene expression signatures following nerve injury in a rat model (0d-14d)

Project description:We used microarrays to distinguish the gene expression differences among different time points after injury. We generated L4-6 dorsal root ganglia (DRG) tissues and proximal sciatic nerve (SN) tissues (0.5cm) at 0d, 1d, 4d, 7d and 14d after sciatic nerve resection.

2013-01-01 | E-GEOD-30165 | biostudies-arrayexpress

Rattus norvegicus

Project description:Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [proximal sciatic nerve]

| PRJNA136341 | ENA

Entrapment Neuropathy Results in Different MicroRNAs Expression Patterns from Denervation Injury in Rats

Project description:To compare the microRNAs (miRNAs) expression profile in the innervated soleus muscle and L4-L6 DRG neuronsafter sciatic nerve entrapment with a non-constrictive silastic tube, subsequent surgical decompression, and denervation injury. The experimental soleus muscles and dorsal root ganglions (DRGs) from each experimental group (sham control, denervation, entrapment, and decompression) were analyzed with an Agilent® rat miRNA array to detect dysregulated miRNAs Three-condition experiment, DRGs and soleus muscles of the rats receiving sciatic nerve denervation 6 months, sciatic nerve entrapment 6 months, and sciatic nerve entrapment 6 months then decompression for 3 months v.s. soleus muscle (sham control), Biological replicates: 1 control replicates, 3 experiment replicates

2010-06-12 | E-GEOD-22181 | biostudies-arrayexpress

Signaling to Transcription Networks in the Neuronal Retrograde Injury Response

Project description:Retrograde signaling from axon to soma activates intrinsic regeneration mechanisms in lesioned peripheral sensory neurons; however, the links between axonal injury signaling and the cell body response are not well understood. Here, we used phosphoproteomics and microarrays to implicate ~900 phosphoproteins in retrograde injury signaling in rat sciatic nerve axons in vivo and ~4500 transcripts in the in vivo response to injury in the dorsal root ganglia. Computational analyses of these data sets identified ~400 redundant axonal signaling networks connected to 39 transcription factors implicated in the sensory neuron response to axonal injury. Experimental perturbation of individual overrepresented signaling hub proteins, including Abl, AKT, p38, and protein kinase C, affected neurite outgrowth in sensory neurons. Paradoxically, however, combined perturbation of Abl together with other hub proteins had a reduced effect relative to perturbation of individual proteins. Our data indicate that nerve injury responses are controlled by multiple regulatory components, and suggest that network redundancies provide robustness to the injury response Microarrays were run on mRNA extracted from adult rat L4 and L5 DRGs cells after 1,3,8,12,16,18,24, and 28 hours after a sciatic nerve (proximal and distal) lesion.

2010-12-29 | E-GEOD-26350 | biostudies-arrayexpress

Blackface Teladorsagia Solexa

Project description:Blackface Teladorsagia Solexa

| PRJEB2335 | ENA

Gene expression signatures following nerve injury in a rat model (0.5h-9h)

Project description:We used microarrays to distinguish the gene expression differences among different time points after injury We generated L4-6 dorsal root ganglia (DRG) tissues (0.5cm) at 0.5h, 3h, 6h and 9h after sciatic nerve resection

2015-08-01 | E-GEOD-65053 | biostudies-arrayexpress

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