Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Dual mitotic bookmarking by GAF condensates and H3K27ac orchestrates differential propagation of cell fate memory in neural development [CUT&Tag]

ABSTRACT: In brain development, neural stem cells (NSCs) undergo asymmetric cell divisions to replicate themselves and meanwhile produce differentiating siblings. It remains obscure how NSCs preserve their self-renewing fate across mitosis. Even less is known how cell fate memory is differentially propagated to sibling daughter cells adopting distinct cell fates. Here we found that key differentiation genes are dually bookmarked by pioneer factor GAF (GAGA factor) and H3K27ac in asymmetrically-dividing Drosophila central brain NSCs. In daughter cells adopting NSC fate, GAF promotes self-renewal through timely inhibiting differentiation genes via HDAC1-mediated H3K27 deacetylation, whereas in sibling daughter cells adopting a differentiating fate, GAF occupancy is replaced by competitor SWI/SNF complex, allowing H3K27ac deposition retained and fast activation of differentiation genes. Thus, our study unveils a new paradigm by which cell fate memory can be differentially transmitted to sibling daughter cells via dual antagonistic mitotic bookmarking and selective molecular competition mechanism.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE270072 | GEO | 2025/07/14

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Dual mitotic bookmarking by GAF condensates and H3K27ac orchestrates differential propagation of cell fate memory in neural development [ATAC-seq]

Project description:In brain development, neural stem cells (NSCs) undergo asymmetric cell divisions to replicate themselves and meanwhile produce differentiating siblings. It remains obscure how NSCs preserve their self-renewing fate across mitosis. Even less is known how cell fate memory is differentially propagated to sibling daughter cells adopting distinct cell fates. Here we found that key differentiation genes are dually bookmarked by pioneer factor GAF (GAGA factor) and H3K27ac in asymmetrically-dividing Drosophila central brain NSCs. In daughter cells adopting NSC fate, GAF promotes self-renewal through timely inhibiting differentiation genes via HDAC1-mediated H3K27 deacetylation, whereas in sibling daughter cells adopting a differentiating fate, GAF occupancy is replaced by competitor SWI/SNF complex, allowing H3K27ac deposition retained and fast activation of differentiation genes. Thus, our study unveils a new paradigm by which cell fate memory can be differentially transmitted to sibling daughter cells via dual antagonistic mitotic bookmarking and selective molecular competition mechanism.

2025-07-14 | GSE292192 | GEO

Dual mitotic bookmarking by GAF condensates and H3K27ac orchestrates differential propagation of cell fate memory in neural development [RNA-seq]

2025-07-14 | GSE270071 | GEO

Expression profiling of prospero mutant VNC cells in Drosophila embryos

Project description:Paper abstract: Stem cells have the remarkable ability to give rise to both self-renewing and differentiating daughter cells. Drosophila neural stem cells segregate cell-fate determinants from the self-renewing cell to the differentiating daughter at each division. Here, we show that one such determinant, the homeodomain transcription factor Prospero, regulates the choice between stem cell self-renewal and differentiation. We have identified the in vivo targets of Prospero throughout the entire genome. We show that Prospero represses genes required for self-renewal, such as stem cell fate genes and cell-cycle genes. Surprisingly, Prospero is also required to activate genes for terminal differentiation. We further show that in the absence of Prospero, differentiating daughters revert to a stem cell-like fate: they express markers of self-renewal, exhibit increased proliferation, and fail to differentiate. These results define a blueprint for the transition from stem cell self-renewal to terminal differentiation. 6 wildtype samples and 6 prospero mutant samples were hybridised to arrays against a commom reference sample (generated from whole embryo cDNA). The prospero samples were indirectly compared to the wildtype samples via the common reference. Half the wildtype and half of the prospero arrays were dye-swapped.

2010-06-25 | E-GEOD-18213 | biostudies-arrayexpress

Expression profiling of prospero mutant VNC cells in Drosophila embryos

2009-10-01 | GSE18213 | GEO

BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (RNA-seq II)

Project description:We found cBAF and c-Myc co-assort asymmetrically to the two daughter nuclei, such that daughter cells with low c-Myc and cBAF display a cell fate trajectory towards memory T cell differentiation. Moreover, cBAF complex and c-Myc physically interact with each other, and act in establishing the chromatin landscape in activated CD8 T cells.

2022-04-24 | GSE199184 | GEO

BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (ATAC III)

2022-04-24 | GSE198894 | GEO

BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (Microarray)

2022-04-24 | GSE184587 | GEO

BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (ATAC II)

2022-04-24 | GSE183618 | GEO

BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (Cut&Run)

2022-04-24 | GSE183617 | GEO

BAF complex cooperates with c-Myc to orchestrate early fate decisions of CD8 T cells (ATAC I)

2022-04-24 | GSE183616 | GEO