Genomics

Dataset Information

0

Multiway chromatin conformation analyses at the human immunoglobulin heavy chain locus reveal regulatory principles underlying somatic hypermutation [ATAC-Seq]


ABSTRACT: The generation of protective antibodies by somatic hypermutation (SHM) is essential for antibody maturation and adaptive immunity. SHM involves co-transcriptional mutagenesis of immunoglobulin variable (V) regions regulated by enhancers located hundreds of kilobases away. How 3D chromatin structure affects SHM is poorly understood. Here, we measure higher-order interactions on single alleles of the human immunoglobulin heavy chain locus (IGH) using Tri-C. We find that SHM is underpinned by a multiway hub wherein the V region is proximal to all enhancers. Cohesin-mediated loop extrusion is dispensable for IGH transcription and hub architecture. Transcription and mutagenesis of IGH switch regions, which are necessary for antibody class-switch recombination, creates new chromatin loops that can form without cohesin. However, these additional loops do not compromise hub integrity, V region transcription or SHM. Thus, antibody maturation occurs within a multiway hub accommodating several gene-enhancer loops wherein transcription and mutagenesis of different segments can occur non-competitively.

ORGANISM(S): Homo sapiens

PROVIDER: GSE271541 | GEO | 2025/06/16

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
GSE271541_RAW.tar Raw
filelist.txt Txt
Items per page:
1 - 3 of 3

Similar Datasets

2025-06-20 | GSE273433 | GEO
2025-06-16 | GSE273431 | GEO
2025-06-20 | GSE271544 | GEO
2025-06-16 | GSE271542 | GEO
2013-10-11 | E-GEOD-43594 | biostudies-arrayexpress
2013-10-11 | GSE43594 | GEO
2021-07-29 | GSE180899 | GEO
2018-10-04 | GSE118794 | GEO
2019-12-04 | GSE139810 | GEO
2020-07-01 | GSE140975 | GEO