Transcriptional identity of murine healthy spleenic, tumor-bearing splenic and tumoral Treg cells.

ABSTRACT: CRC-organoids were implanted in the liver of Foxp3eGFP mice to investigate the phenotypic differences of TI-Treg cells in vivo dependent on location. After tumor development, T cells from distinct metastatic sites were isolated and analyzed. RNA sequencing of Treg cells from the spleen, "primary" liver tumors, and metastases revealed that TI-Treg cells expressed core genes previously associated with CRC-related Treg cells, with subtle location-specific variations. Gene Ontology analysis highlighted enriched immune response regulation pathways in primary liver TI-Treg cells compared to healthy splenic Treg cells. High expression of genes upregulated in liver TI-Treg cells was associated with poor CRC prognosis. Splenic Treg cells from tumor-bearing mice also displayed distinct transcriptional profiles from their healthy counterparts. These findings highlight that TI-Treg cells have transcriptional profiles linked to poor CRC prognosis, with subtle location-specific variations.

ORGANISM(S): Mus musculus

PROVIDER: GSE275756 | GEO | 2026/08/20

REPOSITORIES: GEO