Dataset Information


Determinants of heat resistance in Escherichia coli AW1.7

ABSTRACT: Microarray hybridization of cDNA libraries obtained from exponentially growing or heat-shocked AW1.7 or GGG10 cultures was performed to compare gene expression of these two strains. Expression of selected genes from different functional groups was quantified by quantitative PCR (q-PCR). DnaK, 30S and 50S risobomal subunits were overexpressed in E. coli GGG10 relative to E. coli AW1.7 upon heat shock at 50°C, indicating improved ribosome stability. The outer membrane porin NmpC and several transport proteins were overexpressed in exponentially growing E. coli AW1.7. Overall design: Gene expression of a heat resistant strain, E. coli AW1.7, was compared to gene expression in a heat sensitive strain, E coli GGG10. RNA was isolated from late exponential cultures, or from late exponential cells heat-shocked by exposure to 50°C for 15 min. Three independent biological repeats were analyzed, and technical repeats (dye-swap) were performed for two of three biological repeats.

INSTRUMENT(S): University of Alberta Escherichia coli 3x6K microarray

ORGANISM(S): Escherichia coli  

SUBMITTER: Michael Gänzle 

PROVIDER: GSE27607 | GEO | 2011-03-02



Similar Datasets

2011-03-02 | E-GEOD-27607 | ArrayExpress
| PRJNA261257 | ENA
2015-09-08 | E-GEOD-19569 | ExpressionAtlas
| GSE61749 | GEO
2018-07-18 | GSE107973 | GEO
| GSE52770 | GEO
2013-10-31 | E-GEOD-51870 | ArrayExpress
2012-09-24 | E-GEOD-24007 | ArrayExpress
| GSE24007 | GEO
| GSE102091 | GEO