Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Anti-Flag, anti-H3K4me3 and anti-H3K27ac Cut&Run on DU145 cells with endogenous BRD4 knockout and re-introduced of Flag-tagged WT- or AA-BRD4

ABSTRACT: In this experiment, we performed anti-Flag, anti-H3K4me3 and anti-H3K27ac Cut&Run on DU145 cells with endogenous BRD4 knockout and re-introduced of Flag-tagged WT- or AA-BRD4.

ORGANISM(S): Homo sapiens

PROVIDER: GSE276952 | GEO | 2026/09/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

FLAG-tagged FOXM1A Plasmid

Project description:FLAG-tagged FOXM1A Plasmid

| PRJEB24606 | ENA

iCLIP of FLAG-PURA overexpression with anti-FLAG antibody in HeLa cells

Project description:iCLIP of FLAG-PURA overexpression with anti-FLAG antibody in HeLa cells

| PRJNA798375 | ENA

ChIP-seq of MOZ (Flag-V5-BIO tagged) in mouse mouse craniofacial development

Project description:Carboxy-terminally tagged MOZ (Flag-V5-BIO tagged) was detected by ChIP-seq using anti-V5 antibody (Sigma, A7345) to precipitate chromatin associated with MOZ

2019-07-23 | GSE134607 | GEO

Chip-chip from C-33 cells expressing FLAG-HPV1 E2 with FLAG-HPV1 E2

Project description:HPV1 E2 binds with Brd4 to host mitotic chromosomes. To identify the targets, chromatin was prepared from mitotic C-33 cells expressing HPV1 E2, and analyzed by ChIP-chip analysis for binding to a portion of the human genome. E2 and Brd4 bind to active promoters in interphase C-33 cells (Jang et al., 2009), however, in mitosis E2 was observed instead to bind to a few extremely broad peaks on several chromosomes. These peaks ranged in size from several hundred Kb to >1 Mb and, for the most part, overlapped coding regions. Signals of anti-FLAG E2 ChIP DNA from mitotic HPV1 E2 expressing C-33 cells

2014-04-14 | E-GEOD-52312 | biostudies-arrayexpress

Brd4 and JMJD6-associated Anti-pause Enhancers in Regulation of Transcriptional Pause Release

Project description:Distal enhancers characterized by H3K4me1 mark play critical roles in developmental and transcriptional programs. However, potential roles of specific distal regulatory elements in regulating RNA Polymerase II (Pol II) promoter-proximal pause release remain poorly investigated. Here we report that a unique cohort of jumonji C domain-containing protein 6 (JMJD6) and bromodomain-containing protein 4 (Brd4) co-bound distal enhancers, termed anti-pause enhancers (A-PEs), regulate promoter-proximal pause release of a large subset of transcription units via long-range interactions. Brd4-dependent JMJD6 recruitment on A-PEs mediates erasure of H4R3me2(s), which is directly read by 7SK snRNA, and decapping/demethylation of 7SK snRNA, ensuring the dismissal of the 7SKsnRNA/HEXIM inhibitory complex. The interactions of both JMJD6 and Brd4 with the P-TEFb complex permit its activation and pause release of regulated coding genes. The functions of JMJD6/ Brd4-associated dual histone and RNA demethylase activity on anti-pause enhancers have intriguing implications for these proteins in development, homeostasis and disease. All Gro-seq(s) were designed to reveal the transcriptional targets of JMJD6 and Brd4, and assess the role of JMJD6 and Brd4 in Pol II promoter-proximal pause release. All ChIP-seq(s) were designed to understand the unique features, associated molecular mechanisms and functions of the anti-pause enhancers (A-PEs) discovered in the current study.

2014-01-14 | E-GEOD-51633 | biostudies-arrayexpress

Genome-wide maps of chromatin state in HCT116 cells expressing Flag-tagged VCP

Project description:Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for VCP and Flag in HCT116 cells expressing Flag-tagged VCP.

2023-07-18 | GSE201200 | GEO

Purification of endogenously FLAG-HA-tagged KAT6B from E14 ESCs

Project description:An N-terminal FLAG-HA tag was introduced into the endogenous Kat6b locus by CRISPR genome editing and purified by FLAG affinity purification using anti-FLAG agarose beads.

2025-01-02 | PXD015988 | Pride

FLAG-tagged hnRNPM iCLIP in AGS cells

Project description:FLAG-tagged hnRNPM iCLIP in AGS cells

| PRJNA737793 | ENA

Anti-FLAG-affinity purification of Umbrea associated factors

Project description:Anti-FLAG-affinity purification of Umbrea associated factors from nuclear extracts of Drosophila Schneider cells expressing FLAG-HA-Umbrea. Anti-FLAG -affinity purifications from Schneider cell nuclear extracts not expressing any FLAG-tagged protein served as a control SDS-PAGE separting both samples, 9 slices per sample, reduction and alkylation, digestion with trypsin. LC-MS on a Ultimate3000-LTQ Orbitrap XL Raw data were analysed using the MaxQuant 1.2.2.5 software package. Identified proteins were considered as interaction partners if their MaxQuant intensities displayed a greater than 10 fold enrichment compared to control anti-FLAG purifications from L2-4 nuclear extracts not expressing any FLAG-tagged protein

2013-10-03 | PXD000163 | Pride

Differentially expressed genes in cells expressing WT or phospho-inactivating mutant (AA) BRD4

Project description:In this experiment, we use RNA-seq to examine the differentially expressed genes in DU145 and PC3 cells expressing WT (BRD4-WT) or phospho-inactivating mutant BRD4 (BRD4-AA)

2026-09-28 | GSE289083 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data