Anti-FLAG-affinity purification of Umbrea associated factors

ABSTRACT: Anti-FLAG-affinity purification of Umbrea associated factors from nuclear extracts of Drosophila Schneider cells expressing FLAG-HA-Umbrea. Anti-FLAG -affinity purifications from Schneider cell nuclear extracts not expressing any FLAG-tagged protein served as a control SDS-PAGE separting both samples, 9 slices per sample, reduction and alkylation, digestion with trypsin. LC-MS on a Ultimate3000-LTQ Orbitrap XL Raw data were analysed using the MaxQuant 1.2.2.5 software package. Identified proteins were considered as interaction partners if their MaxQuant intensities displayed a greater than 10 fold enrichment compared to control anti-FLAG purifications from L2-4 nuclear extracts not expressing any FLAG-tagged protein

INSTRUMENT(S):

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Andreas Thomae  

LAB HEAD: Andreas Thomae

PROVIDER: PXD000163 | Pride | 2013-10-03

REPOSITORIES: Pride