CUT&RUN analysis of H3K37me1 and H3K9me3 in fibroblasts treated with TNFa ± TPCA1
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ABSTRACT: Analysis of changes in chromatin modifications H3K37me1 and H3K9me3 in fibroblast cell line GM02036 (Coriell) after treatment with TNFa and/or TPCA1.
Project description:Analysis of changes in chromatin modifications H3K9me1 and H3K27me3 in fibroblast cell line GM02036 (Coriell) after treatment with TNFa and/or TPCA1.
Project description:Analysis of changes in chromatin modifications H3K36me3 and H4K20me1 in fibroblast cell line GM02036 (Coriell) after treatment with TNFa and/or TPCA1.
Project description:Analysis of changes in chromatin modifications H3K27ac and H3K4me1 in fibroblast cell line GM02036 (Coriell) after treatment with TNFa and/or TPCA1.
Project description:Analysis of changes in chromatin binding of RelA and MCM2 in fibroblast cell line GM02036 (Coriell) after treatment with TNFa and/or TPCA1.
Project description:Analysis of changes in chromatin binding of RelA in fibroblast cell line GM02036 (Coriell) after treatment with viral mimetic polyinosinic:polycytidylic acid (poly(I:C)) ± TPCA1.
Project description:Analysis of changes in chromatin binding of STAT1 and IRF3 in fibroblast cell line GM02036 (Coriell) after treatment with viral mimetic polyinosinic:polycytidylic acid (poly(I:C)) ± TPCA1.
Project description:Analysis of changes in chromatin modifications H3K27ac and H3K4me1 in fibroblast cell line GM02036 (Coriell) ± heterozygous deletion of 259 bp region on Chr21 (33204248-33204506) after treatment with TNFa.
Project description:Analysis of changes in chromatin binding of RelA and MCM2 in fibroblast cell line GM02036 (Coriell) ± heterozygous deletion of 259 bp region on Chr21 (33204248-33204506) after treatment with TNFa.
Project description:Analysis of the RNA-seq data performed in IR vs NIR hematopoietic stem cells show the loss of the TNF_via_NFKB signature. We showed that the loss of this signature could be associated with H3K9me3 loss at specific retrotransposable elements . To validate this association, we tested if TNFa treatment before irradiation was able to prevent IR-effect on H3K9me3 loss at retrotransposable elements. For this purpose, we treated mice with TNFa 1h before irradiation (IR_TNF) and performed H3K9me3 cut&tag experiments on hematopoietic stem cells 1 month after irradiation and compared them to hematopoietic stem cells sorted from non irradiated mice (NIR) and from non-treated irradiated mice (IR).
Project description:Spermatogenesis is a recurring differentiation process that results in the production of male gametes within the testes. During this process, spermatogonial stem cells differentiate to form spermatocytes, which undergo two rounds of meiotic division to form haploid spermatids. Throughout spermiogenesis, round spermatids elongate to form mature sperm. To profile changes in chromatin marks between spermatocytes and spermatids, we generated CUT&RUN data of H3K4me3, H3K27ac and H3K9me3 marks in sorted spermatocytes and spermatids.